Inheritance of TSWV resistance in a new tobacco (Nicotiana tabacum L.) breeding line
Dorota Laskowska
iung@iung.pulawy.plInstytut Uprawy Nawożenia i Gleboznawstwa — Państwowy Instytut Badawczy w Puławach (Poland)
Abstract
Tomato spotted wilt virus (TSWV) has been reported worldwide in a 1090 different host species. The most effective way to minimize the damage of this disease is to develop resistance of the field crops. The tobacco breeding line developed in IUNG-PIB in Puławy exhibits a high level of TSWV resistance. The objective of this study was to investigate the inheritance of the resistance present in the L-9 line. The resistant parental L-9 line and susceptible cultivar Wiślica were crossed in both directions to create F1 hybrids, from which backcrosses with resistant and susceptible parent were made and F2 generation were obtained. All populations were evaluated for response to artificial inoculation with one TSWV isolate of local origin, in greenhouse conditions. The presence of the virus was verified using DAS-ELISA. A dominant character and no material effect were stated for the resistance trait. Segregation ratios of resistant and susceptible phenotypes in the testing crosses were in agreement with the hypothesis that inheritance of TSWV resistance of the L-9 line is dominant and monogenic.
Keywords:
Nicotiana tabacum L., Tomato spotted wilt virus, disease resistance gene, tobacco, inheritanceReferences
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Authors
Dorota Laskowskaiung@iung.pulawy.pl
Instytut Uprawy Nawożenia i Gleboznawstwa — Państwowy Instytut Badawczy w Puławach Poland
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