Application of the RAPD and SSR methods to genetic similarity assessment of tetraploid species of the Avena L. genus
Edyta Paczos-Grzęda
edyta.paczos@up.lublin.plInstytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie (Poland)
Abstract
Tetraploids of the genus Avena L.: A. maroccana, A. murphyi and A. macrostachya were evaluated for genetic similarity and relatedness with the hexaploid species A. sativa and A. sterilis based on RAPD and SSR polymorphism. Fifteen RAPD primers produced 129 polymorphic DNA fragments and 13 SSR primer pairs amplified 141 products of such type. Polymorphism information content (PIC) values ranged from 0.58 to 0.80 for RAPD and from 0.71 to 0.92 for SSR. Mean values of PIC for RAPD was 0.67 and for SSR — 0.82. Dice genetic similarity indices were used for cluster analysis with the UPGMA method. The AACC genome tetraploids clustered together with the AACCDD genome hexaploids. Simultaneously, AACC tetraploids: A. maroccana and A. murphyi were found to form two separate subclusters. The analyzed genotypes of the CCCC autotetraploid A. macrostachya formed an outer branch, indicating a major genomic divergence. Topology of both constructed dendrograms was the same and consistent with the Avena L. genus systematics.
Keywords:
Avena L., RAPD, SSR, podobieństwo genetyczneReferences
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Authors
Edyta Paczos-Grzędaedyta.paczos@up.lublin.pl
Instytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie Poland
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