Cry1Ab expression in MON810 maize varieties in Poland — impact on non-target organisms the grain aphid (Sitobion avenae F.) and the bird cherry-oat aphid (Rhopalosiphum padi L.)
Anna Linkiewicz
a.linkiewicz@ihar.edu.plLaboratorium Kontroli GMO, Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie (Poland)
Magdalena Żurawska-Zajfert
Laboratorium Kontroli GMO, Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie (Poland)
Zbigniew T. Dąbrowski
Katedra Entomologii Stosowanej, Wydział Ogrodniczy Szkoły Głównej Gospodarstwa Wiejskiego (Poland)
Sławomir Sowa
Laboratorium Kontroli GMO, Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie (Poland)
Abstract
The transgenic MON810 maize (Zea mays L.), expressing Cry1Ab protein and known to have the most effective protection against European corn borer, has been cultivated in Poland since 2007. MON810 cultivars express 92 kDA N-terminal fragment of Cry1Ab protein from Bacillus thuringiensis (Bt) ssp. kurstaki strain HD1, assuring protection against Lepidoptera insects. Possible unintended effects of the widespread planting of Bt crops (insect resistance, impact on non-target organisms) are related to the expression level of Bt protein. Non-target herbivores and beneficial insects may be affected by direct feeding on the transgenic crop or by interactions in tri-trophic systems. In MON810 plants the Bt protein expression should have constitutive character due to the presence of 35S promoter. However, considerable variations in the expression level of Cry1Ab protein have been reported. A quantitative Enzyme-Linked Immunosorbent Assay (ELISA) was used to quantify the levels of Cry1Ab protein in DKC60-16Bt and DKC3421YG cultivars grown under greenhouse and field conditions. The tissue-specific expression and cultivation-dependent abundance of Cry1Ab protein were determined. The accumulation of Bt protein in selected non-target organisms is discussed.
Supporting Agencies
Keywords:
MON810, aphids, Cry1Ab expression, non-target species, risk assessmentReferences
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Authors
Anna Linkiewicza.linkiewicz@ihar.edu.pl
Laboratorium Kontroli GMO, Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie Poland
Authors
Magdalena Żurawska-ZajfertLaboratorium Kontroli GMO, Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie Poland
Authors
Zbigniew T. DąbrowskiKatedra Entomologii Stosowanej, Wydział Ogrodniczy Szkoły Głównej Gospodarstwa Wiejskiego Poland
Authors
Sławomir SowaLaboratorium Kontroli GMO, Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie Poland
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Copyright (c) 2009 Anna Linkiewicz, Magdalena Żurawska-Zajfert, Zbigniew T. Dąbrowski, Sławomir Sowa
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