The diagnosis of latent form of potato ring rot (Clavibacter michiganensis subsp. sepedonicus). Pathogenicity test on eggplants (Solanum melongena L.)
Teresa Pastuszewska
t.pastuszewska@ihar.edu.plPracownia Chorób i Szkodników Kwarantannowych Ziemniaka, Instytut Hodowli i Aklimatyzacji Roślin, Oddział w Bydgoszczy (Poland)
Sebastian Brzozowski
Pracownia Chorób i Szkodników Kwarantannowych Ziemniaka, Instytut Hodowli i Aklimatyzacji Roślin, Oddział w Bydgoszczy (Poland)
Abstract
The development of bacterial ring rot symptoms was observed on eggplants which were inoculated with different amounts of Clavibacter michiganensis subsp. sepedonicus (Cms) and usefulness of the bioassay in eggplant seedlings was assessed for verification of the results of indirect immunofluorescence antibody staining (IFAS). Plants infected with the suspensions of bacterial cells which contained from 10 colony forming units (cfu)·ml-1 to 4.8 × 102 cfu·ml-1 not show any disease symptoms for over 40 days. In all cases the IFAS-test confirmed the latent infection (Table 1 and 2). The symptoms were observed on plants infected with the Cms suspension in the concentration above 9.1 × 103 cfu·ml-1. The first symptoms were observed 7 days after inoculation, on 4 from 10 plants inoculated with the highest concentration inoculum (9.1 ×106 cfu·ml-1): however 13 days later the symptoms were visible on all eggplants.
Keywords:
bacterial ring rot of potato, bioassay, eggplant, IFAS-testReferences
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Authors
Teresa Pastuszewskat.pastuszewska@ihar.edu.pl
Pracownia Chorób i Szkodników Kwarantannowych Ziemniaka, Instytut Hodowli i Aklimatyzacji Roślin, Oddział w Bydgoszczy Poland
Authors
Sebastian BrzozowskiPracownia Chorób i Szkodników Kwarantannowych Ziemniaka, Instytut Hodowli i Aklimatyzacji Roślin, Oddział w Bydgoszczy Poland
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