Genetic analysis of several quantitative traits linked with regeneration of false flax (Camelina sativa L.) in in vitro tissue cultures
Tadeusz Łyuczkiewicz
agro@up.poznan.plKatedra Genetyki i Hodowli Roślin, Akademia Rolnicza im. A. Cieszkowskiego w Poznaniu (Poland)
Jerzy Nawracała
Katedra Genetyki i Hodowli Roślin, Akademia Rolnicza im. A. Cieszkowskiego w Poznaniu (Poland)
Małgorzata Strybe
Katedra Genetyki i Hodowli Roślin, Akademia Rolnicza im. A. Cieszkowskiego w Poznaniu (Poland)
Karolina Satkiewicz
Katedra Genetyki i Hodowli Roślin, Akademia Rolnicza im. A. Cieszkowskiego w Poznaniu (Poland)
Abstract
Diallel crosses (Griffing’s type II) between six different genotypes of false flax (Camelina sativa L.) originated from European collections were done. Explants (hypocotyl-cotyledon parts) from F1 hybrids and parental plants were placed on induction medium (MS + 1 mg/l BAP). After 8 weeks of the in vitro culture maintenance, a number of shoots regenerated from each explant, number of leaves per explant, length of shoots as well as weight of callus and of the regenerated parts of plant were determined. It was found that the shoots regenerated by indirect organogenesis process. From one explant 2.8–5.8 shoots were regenerated. The highest number of shoots were obtained with hybrid plants of the line 4 × line 13 cross. The longest shoots (4.3 cm) were regenerated from the explants of line 15. Analysis of general combining ability showed that line 15 affected in hybrid plants the increase in length of regenerated shoots, line 13 — the increase in number of leaves on shoots, and line 9 — the decrease in weight of callus and of the regenerated parts of plant. Heritability both in broad and narrow sense was very low: 0.57 and 0.04 for the length of shoots, and 0.38 and 0.15 for the number of leaves per shoot, respectively. Effects of domination were found to be significant for the length of shoots.
Keywords:
false flax, in vitro culture, general combining ability, heritabilityReferences
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Authors
Tadeusz Łyuczkiewiczagro@up.poznan.pl
Katedra Genetyki i Hodowli Roślin, Akademia Rolnicza im. A. Cieszkowskiego w Poznaniu Poland
Authors
Jerzy NawracałaKatedra Genetyki i Hodowli Roślin, Akademia Rolnicza im. A. Cieszkowskiego w Poznaniu Poland
Authors
Małgorzata StrybeKatedra Genetyki i Hodowli Roślin, Akademia Rolnicza im. A. Cieszkowskiego w Poznaniu Poland
Authors
Karolina SatkiewiczKatedra Genetyki i Hodowli Roślin, Akademia Rolnicza im. A. Cieszkowskiego w Poznaniu Poland
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Copyright (c) 2006 Tadeusz Łyuczkiewicz, Jerzy Nawracała, Małgorzata Strybe, Karolina Satkiewicz
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