The use of marker-assisted selection for introduction of leaf rust resistance gene Lr50 (Puccinia recondita f. sp. tritici) to wheat
Paweł Cz. Czembor
p.czembor@ihar.edu.plInstytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Aleksandra Pietrusińska
Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Abstract
The leaf rust resistance gene Lr50 was introduced to the wheat variety Flair using marker-assisted selection strategy. Two backcrosses to the recurrent parent were performed. Two microsatellites gwm382 and gdm87 flanking gene Lr50 were used to select individuals with the resistance gene in offspring populations. In addition, recipient genome content was evaluated on F1BC1 population using a set of 21 microsatellite markers. Therefore, in the centromer region of each chromosome one marker was located. Unfortunately, due to the lack of DNA polymorphism or absence of expected PCR products, the information obtained was limited. Nevertheless, the marker-assisted selection strategy allowed to select lines carrying gene Lr50. The lines were transferred to the wheat breeding program focused on the resistance to leaf rust.
Keywords:
leaf rust, marker-assisted selection, resistance, wheatReferences
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Authors
Paweł Cz. Czemborp.czembor@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Aleksandra PietrusińskaInstytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
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