Cytogenetic and molecular analyses of interspecific hybrids of the hexaploid oat Avena sativa L. × Avena sterilis L. and initial forms
Edyta Paczos-Grzęda
genetyka.roslin@up.lublin.plInstytut Genetyki i Hodowli Roślin, Akademia Rolnicza, Lublin (Poland)
Abstract
The RAPD (Random Amplified Polymorphic DNA) method was used to confirm the hybrid character of Avena sativa L. × Avena sterilis L. crosses. DNA fragments amplified in the hybrids and paternal forms, but not observed in cultivars, were identified. Configurations of chromosomes at metaphase I, frequency of lagging chromosomes and chromatid bridges at anaphase I, occurrence of micronuclei at tetrads, viability and size of pollen grains in the hybrids and parental forms were analyzed. Univalents and multivalents were not observed at metaphase I PMC. Bivalents were generally of ring shape and occasionally of rod. The hybrids, as compared with parental forms, were characterized by the higher frequencies of lagging chromosomes and chromatid bridges. Tetrads without micronuclei predominated in all the analyzed forms. Interspecific crosses, except Dragon × A. sterilis, had a greater number of micronuclei per tetrad than initial forms. Despite the differences in the occurrence of lagging chromosomes, chromatid bridges and tetrads with micronuclei, the pollen viability in the examined material was very high.
Keywords:
Avena sativa L., Avena sterilis L., RAPD, interspecific hybrids, meiosisReferences
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Authors
Edyta Paczos-Grzędagenetyka.roslin@up.lublin.pl
Instytut Genetyki i Hodowli Roślin, Akademia Rolnicza, Lublin Poland
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