Evaluation of genetic variation of inbred lines and a degree of hybridity of rye by SDS-PAGE method
Miłosz Smolik
dziekanat.wksir@zut.edu.plAkademia Rolnicza w Szczecinie (Poland)
Danuta Rzepka-Plevneš
Akademia Rolnicza w Szczecinie (Poland)
Abstract
The object of the studies were 14 F1 hybrids obtained by crossing 13 inbred lines of different tolerance to nutrient deficiency in the medium at a seedling stage. Parental lines originated from the collection of: the Department of Plant Breeding of Szczecin Agricultural University and the Department of Plant Genetics, Breeding and Biotechnology of Warsaw Agricultural University, and F1 hybrids originated from our earlier studies. Genotypes of F1 hybrids and their parental forms were compared on basis of analysis of storage proteins using SDS-PAGE test (discontinuous system) performed on two kinds of polyacrylamide gels (4 and 12.5%) in Mini Protein II apparatus at constant voltage 50 mA. The obtained electrophoretic patterns were analyzed by means of the computer programme Diversity one 1.3” to evaluate the number and weight of bands in the light of a scanner Sharp JX-330. It was found that despite phenotypic differences (tolerance level) inbred lines belonged to the same group of genetic similarity. Analysis of electrophoretic variation of secalin in F1 hybrids obtained by crossing inbred lines showed the presence of protein markers of both parental components the great majority of hybrids. The hybrids, in which maternal bands dominated (genetic similarity 61–94%) were prevalent. The results indicate that SDS-PAGE test may be used to evaluation of a degree of rye varieties hybridity.
Keywords:
SDS-PAGE, inbred lines, F1 hybrids, polymorphismReferences
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Authors
Danuta Rzepka-PlevnešAkademia Rolnicza w Szczecinie Poland
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