Cysteine endoproteinases activity as an indicator of wheat resistance to frost and drought
Małgorzata Grudkowska
m.grudkowska@ihar.edu.plInstytut Hodowli i Aklimatyzacji Roślin w Radzikowie (Poland)
Krzysztof Wiśniewski
Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie (Poland)
Barbara Zagdańska
Katedra Biochemii Szkoła Główna Gospodarstwa Wiejskiego w Warszawie (Poland)
Abstract
Frost resistance of seedlings of twenty cultivars and strains of winter wheat was significantly positively correlated with their dehydration tolerance (r = 0.862). Cysteine endoproteinase activity of control seedlings, non-acclimated to low temperature, was not correlated with the level of frost resistance (r = -0.0028). After 10 day acclimation period, cysteine endoproteinase activity in seedlings of frost resistant cultivars remained unchanged or even lowered, but increased in seedlings of susceptible cultivars and was significantly negatively correlated with the level of frost resistance (r = - 0.853). The experiments showed that the extent of cysteine endoproteinase induction reflects the level of resistance of winter wheat to frost and water deficiency in the seedling phase. The described procedure seems to be useful as a simple laboratory test for comparative estimation of the resistance level of breeding materials of winter wheat to the both most common abiotic stresses.
Keywords:
cysteine endoproteinases, drought resistance, frost resistance, winter wheatReferences
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Authors
Małgorzata Grudkowskam.grudkowska@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie Poland
Authors
Krzysztof WiśniewskiInstytut Hodowli i Aklimatyzacji Roślin w Radzikowie Poland
Authors
Barbara ZagdańskaKatedra Biochemii Szkoła Główna Gospodarstwa Wiejskiego w Warszawie Poland
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