Variability of rye populations selected for tolerance to nitrogen deficiency in hydroponic cultures
Danuta Rzepka-Plevneš
dziekanat.wksir@zut.edu.plZakład Hodowli Roślin Ogrodniczych, Akademia Rolnicza w Szczecinie (Poland)
Abstract
Nine rye cultivars, two strains and their progeny S1, S2 obtained after mass selection directed towards increased tolerance to N deficit were used in the study. The selection performed in hydroponic cultures was based on the morphological traits of 7-day seedlings developed for ten days on the medium containing 10.506 mg N/dm3. The control constituted seedlings grown on the Hoagland medium (168.0 mg N/dm3). Each rye population was represented 500 seedlings. The 35–37% of well-developed seedlings considered as tolerant to N deficit, were planted on the plots in the experimental fields. After sib-crossing the collected seeds constituted the initial material for the next cycle of mass selection. Initial material (S0) and S1, S2 populations were compared for their growth characteristics and electrophoretic patterns of storage proteins obtained by SDS-PAGE method. The results showed that differences in seedling height and root length of the parents and progeny were significant already after the first selection cycle. In the second further cycle a continuous lowering of mean values of the mentioned seedling traits and increasing of root number was observed. Among 12 rye populations produced by selection for tolerance to N deficit in the medium the following genotypes deserve special attention: Amilo, Arant, Adar, Dańkowskie Złote, Motto and SMH 92. They represent a valuable source material of desirable genes. Electrophoretic analyses of secalin patterns showed differences between rye genotypes from S1 and S2 generations in comparison with the initial genotypes. Similarity index between analyzed cultivars and S1 generation ranged from 79.3 to 97.4%, S1 and S2 from 90.3 to 99.6% and between cultivars and S2 from 81.1 to 99.4%.
Keywords:
deficit, hydroponic culture, nitrogen, rye, selection, variabilityReferences
Anioł A. 1981. Metody określania tolerancyjności zbóż na toksyczne działanie jonów glinu. Biul. IHAR 143: 3 — 14.
Google Scholar
Bos I., Henninks S.1991. A comparison of several procedures for mass selection in winter rye. II. What are the merits of adjusting phenotypic values? Euphytica 52: 57 — 64.
DOI: https://doi.org/10.1007/BF00037857
Google Scholar
Caradus J. R., Woodfield D .R. 1998. Genetic control of adaptive root characteristics in white clover. Plant and Soil 200: 63 — 69.
DOI: https://doi.org/10.1023/A:1004296707631
Google Scholar
Escribano M. R., Santalla M., Casquero P. H., de Ron A. M. 1998. Patterns of genetic diversity in landraces of common bean (Phaseolus vulgaris L.) from Galicia. Plant Breeding 117: 449 — 456
DOI: https://doi.org/10.1111/j.1439-0523.1998.tb01447.x
Google Scholar
Falconer D. S., 1974. Dziedziczenie cech ilościowych. PWN, Warszawa
Google Scholar
Fernadez C. J., McInes K. J., Cothren J. T. 1996. Water status and leaf area production in water — and nitrogenstressed cotton. Crop Sci. 36: 1224 — 1233.
DOI: https://doi.org/10.2135/cropsci1996.0011183X003600050026x
Google Scholar
Górny A. G. 1992. Genetic variation of the root system on spring barley and oat. Rozprawy Naukowe Instytutu Genetyki PAN, Poznań: 5 — 89.
Google Scholar
Górny A. G., 1993. Differences in root and shoot response to limited N-supply in oat and spring barley. J. Agron. Crop Sci. 171: 161 — 167.
DOI: https://doi.org/10.1111/j.1439-037X.1993.tb00127.x
Google Scholar
Górny A. G. 1995. Cechy korzeni w hodowli roślin o obniżonych wymaganiach pokarmowych. Post. Nauk Rol. 1: 67 — 91.
Google Scholar
Górny A. G., Szołkowska A. 1996. Effects of selection for more vigorous seminal roots in two cross populations of oat (Avena sativa L.). J. Appl. Genet. 37, 4: 331 — 334.
Google Scholar
Hartmann A., Miedaner T., Geiger H. H. 1996. Genetic variability of rye under low nitrogen condition. Vort. Pflanzenzücht. 35:212 — 213.
Google Scholar
Hegde V. S., Singhal N. C. 2000. Identification and cluster analysis of Indian bread wheat varieties by acid PAGE of gliadin marker. Plant Varieties Seeds 13: 1 — 9.
Google Scholar
Klonsky A., Livingston P. 1944. Alternative systems aim to reduce inputs, maintain profits. California Agriculture 48: 5.
DOI: https://doi.org/10.3733/ca.v048n05p34
Google Scholar
Kucharczyk K. 1997. Elektroforetyczne metody analizy białek. Mat. kursu „Metody biologii molekularnej”., Gdańsk :9 — 15.
Google Scholar
Kulpa D. 2000. Badania nad tolerancją żyta (Secale sp.) na niedobory pokarmowe w podłożu i możliwością jej wykorzystania w hodowli. Pr. doktorska, AR, Szczecin.
Google Scholar
Laemmli V. K. 1970. Cleavage of structural proteins during assembly of the head bacteriopha T4. Nature 227: 680 — 685.
DOI: https://doi.org/10.1038/227680a0
Google Scholar
Madden J. P. 1994. Reforming science and education in an effort to make world agriculture more sustainable. Agric. Progress 69: 11 — 24.
Google Scholar
Niedzielski M., Bednarek P. T., Puchalski J. 1996. Zastosowanie elektroforezy białek zapasowych nasion do identyfikacji odmian żyta. Mat. IV Ogólnopol. Symp. Genetyka ilościowa roślin uprawnych., Polanica Zdrój, 22–25. 05. 1996: 49.
Google Scholar
Przybylska J., Zimniak – Przybylska Z., Krajewski P. 2000. Electrophoretic seed albumin patterns in the (Vicia sativa L.) aggregate. J. Appl. Genet. 4 1/3: 267 — 269.
Google Scholar
Radić-Mieble H., Saam C., Hils R., Kling Ch. L., Hesemann C. U. 1998. Characterization of spelt (Triticum spelta L.) forms by gel-electrophoretic analyses of seed storage proteins. III. Comparative analyses of spelt and Central European winter wheat (Triticum aestivum L.) cultivars by SDS-Page and acid-PAGE. Theor. Appl. Genet. 97: 1340 — 1348.
DOI: https://doi.org/10.1007/s001220051027
Google Scholar
Raun W. R., Johnson G. V. 1999. Improving nitrogen use for cereal production. Agron. J. 91: 357 — 363.
DOI: https://doi.org/10.2134/agronj1999.00021962009100030001x
Google Scholar
Rzepka-Plevneš D., Marciniak H., Smiech M. 1997. Ocena tolerancyjności linii wsobnych żyta (S. cereale L.) na niedobory pokarmowe testem in vitro. Biul. IHAR 203: 137 — 146.
Google Scholar
Rzepka-Plevneš D. Tomczak P. 1988. Tolerancyjność różnych genotypów żyta na niedobory pokarmowe w kulturach wodnych. Biul. IHAR 200: 125 — 131.
Google Scholar
Rzepka-Plevneš D. 1999. Variability of tolerance to nitrogen and potassium deficiencies in original (S0) and selected (S1–S3) rye populations, assessed during in vitro cultures. Plant Breed. Seed Sci. 43, 1: 47 — 63.
Google Scholar
Sinclair T. R. 1998. Historical changes in harvest index and crop nitrogen accumulation. Crop Sci. 38: 638 — 643.
DOI: https://doi.org/10.2135/cropsci1998.0011183X003800030002x
Google Scholar
Smolik M. 2001. Zmienność genotypów żyta Secale cereale L. o różnej tolerancyjności na niedobory pokarmowe w podłożu. Praca doktorska. AR, Szczecin: 121 — 130.
Google Scholar
Sperlich D. 1972. Genetyka populacji. PWN, Warszawa.
Google Scholar
Teyker R. H., Moll R. H., Jackson W. A. 1989. Divergent selection among maize seedlings for nitrate uptake. Crop Sci. 29: 879 — 884.
DOI: https://doi.org/10.2135/cropsci1989.0011183X002900040007x
Google Scholar
Waga J. 1991. Zróżnicowanie frakcji białek gliadynowych u wybranych form pszenicy ozimej i jarej. Hod. Rośl. Aklim. 35, 1/2: 59 — 63.
Google Scholar
Young J. E. B., Cook S. K., Green S. M. 1996. Rotations and cropping systems. Aspects Appl. Biology 47: 137 — 144.
Google Scholar
Authors
Danuta Rzepka-Plevnešdziekanat.wksir@zut.edu.pl
Zakład Hodowli Roślin Ogrodniczych, Akademia Rolnicza w Szczecinie Poland
Statistics
Abstract views: 5PDF downloads: 3
License
Copyright (c) 2025 Danuta Rzepka-Plevneš
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Miłosz Smolik, Danuta Rzepka-Plevneš, Katarzyna Grys, Genetic variability of storage proteins in some winter triticale (X Triticosecale Wittmack) varieties , Bulletin of Plant Breeding and Acclimatization Institute: No. 236 (2005): Regular issue
- Miłosz Smolik, Danuta Rzepka-Plevneš, Evaluation of genetic variation of inbred lines and a degree of hybridity of rye by SDS-PAGE method , Bulletin of Plant Breeding and Acclimatization Institute: No. 226/227 (2003): Regular issue
- Agata Markowska, Danuta Rzepka-Plevneš, Electrophoretic variability of storage protein and morphological features of some rye species (Secale sp.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 236 (2005): Regular issue
- Danuta Rzepka-Plevneš, Miłosz Smolik, Karolina Lebiecka, Polymorphism of seed protein in some rye (Secale sp.) forms determined by SDS-PAGE electrophoresis , Bulletin of Plant Breeding and Acclimatization Institute: No. 231 (2004): Regular issue
- Danuta Rzepka-Plevneš, Miłosz Smolik, Marcelina Krupa, Electrophoretic variability of storage proteins in different triticale (X Triticosecale Wittmack) cultivars , Bulletin of Plant Breeding and Acclimatization Institute: No. 226/227 (2003): Regular issue
