Androgenesis from spring barley anthers culture
Katarzyna Kraska
k.kraska@ihar.edu.plInstytut Hodowli i Aklimatyzacji Roślin, Oddział w Krakowie (Poland)
Abstract
Three-years study on androgenesis in anther cultures of 11 cultivars and 5 F1 hybrids of spring barley demonstrated in different androgenesis ability of the investigated forms. The genotype dependent androgenesis ability and the genotype x medium interaction were confirmed. Four induction media (FHG0, FHG1, BAC4, BAC5) and two regeneration media (FHGR1, FHGR2) were applied. In the first year of study the following percentages of regenerated green plants were recorded for the genotypes: F1 16 — 5%, F1 471 — 5%, F1 463 — 1,2% and for the cultivar Haisa — 2%. The remaining genotypes regenerated into albino plants. Among the hybrid genotypes an influence of mother plant on androgenesis ability was observed. In the second year of study all the cultivars showed good androgenesis response (25% responding anthers), however regeneration of calli was poor: only 2 green plants was obtained from the cultivar Olga. In the third year 5 green plants from the cultivar Krona and 1 from the cultivar Olga were obtained. The result of androgenesis in the cultivar Haisa from the first year was not repeated — only albino plants regenerated. Furthermore, among the applied media the FHG0 and BAC5 proved to be more favorable than the FHG1 and BAC4 in androgenesis induction, whereas there were no differences in effect of the regeneration media FHGR1 and FHGR2.
Keywords:
Hordeum vulgare L., androgenesis in vitro, anther culture, barleyReferences
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Authors
Katarzyna Kraskak.kraska@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin, Oddział w Krakowie Poland
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