Pollen fertility restoration in the CMS-Pampa × restorer crosses of rye
Irena Kolasińska
i.kolasinska@ihar.edu.plZakład Genetyki i Hodowli Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Abstract
Pollen fertility restoration of single crosses between male sterile lines (P lines) and restorers (R) was studied in the Plant Breeding and Acclimatization Institute at Radzików. In 1999 pollen fertility of 63 single crosses (21 P lines × 3 R lines) was evaluated in greenhouse and in field. In 2000 pollen fertility of 50 single crosses (10 P lines × 5 R lines) was estimated in the field. The experiments were laid out as a completely randomized block design with 3 replicates and 10 plants per plot. The degree of male sterility/fertility was assessed by visual anther score of single plants (ASP) on a 1–9 scale, restorer indices (RI-1, RI-2) and visual pollen shedding score of plots (PSSP) on a 1–9 scale. "AGRO" computer software was used for statistic analysis. Coefficients of correlation between fertility traits were computed. The analysis of variance showed that the degree of male fertility of single crosses strongly depends on a restorer genotype, a P line genotype as well as on the P R interaction. The restorer and P line genotypes were a major source of variation. A wide range of variation for restorer index was observed among single crosses in the greenhouse and as well as in the field. Restoration index (RI-1) ranged from 0 to 100%. Mean restorer index for P lines ranged from 7,1 to 96,8% in the greenhouse and from 1,5 to 99,4 % in the field. Data presented in this paper clearly indicates, that choice of female components is very important for rye hybrid breeding. The P lines, in which male fertility could be easily restored, should be used for creation of hybrid varieties. P lines with the fertility hard to restore should be used as testers in a restorer breeding. All the restorers tested, provide a sufficient restoration of male fertility of the single crosses with majority of the P lines. The new restorer 330 R led to significantly higher male fertility levels than the other currently used lines. A high, significant correlation was found between all fertility traits estimated in the greenhouse and in the field. So, the simplest method, pollen shedding score of plots, could be successfully used in a breeding programme.
Keywords:
hybrid breeding, male sterility, restoration of pollen fertility, winter ryeReferences
Chen L. W. 1981. Inheritance of male sterility in maize. Hereditas 3/3: 32 — 35.
Google Scholar
Geiger H. H., Schnell F. W. 1970. Cytoplasmic male sterility in rye (Secale cereale L.).Crop Sci. 10: 590 — 593.
DOI: https://doi.org/10.2135/cropsci1970.0011183X001000050043x
Google Scholar
Geiger H. H., Morgenstern K. 1975. Angewandt-genetische Studien zur cytoplasmatischen Pollensterilität bei Winterroggen. Theor. Appl. Genet. 46: 269 — 276.
DOI: https://doi.org/10.1007/BF00281148
Google Scholar
Geiger H. H., Yuan Y., Miedaner T., Wilde P. 1995. Environmental sensitivity of cytoplasmic-genic male sterility (CMS) in Secale cereale L. In: Genetic mechanisms for hybrid breeding. Kück U., Wricke, G. (eds.). Advances in Plant Breeding 18: 7 — 17.
Google Scholar
Geiger H. H., Miedaner T. 1996. Genetic basis and phenotypic stability of male fertility restoration in rye. Vortr. Pflanzenzüchtg. 35: 27 — 38.
Google Scholar
Keydel F., Münzer W., Fischer K. 1979. Interactions between cytoplasmatic male sterile wheat lines and restoration lines. Cereal Res. Commun. 7. 1: 19 — 24.
Google Scholar
Kolasińska I. 1975. Przywracanie płodności pyłku linii męskosterylnych pszenicy (Timopheevi) Triticum aestivum L. Hod. Rośl. Aklim. 19. 3: 223 — 244.
Google Scholar
Kolasińska I. 1996. Pollen fertility restoration in topcross hybrids of winter rye. Vortr. Pflanzenzüchtg. 35: 72 — 74.
Google Scholar
Kolasińska I. 1998. Genetic bases of male fertility restoration in rye. J. Appl. Genet. 39A: 116 — 117.
Google Scholar
Kolasińska I., Węgrzyn S. 2000. Zdolność kombinacyjna przywracania płodności pyłku mieszańców żyta. Biul. IHAR 216, z. 1: 95 — 100.
Google Scholar
Madej L., Osiński R., Jagodziński J. 1996. Ocena płodności mieszańców żyta. Biul. IHAR 195/196: 283 — 290.
Google Scholar
Morgenstern K. 1983. Ausprägung der cytoplasmatisch-genischen Pollensterilität (CMS) bei Roggen in Abhängigkeit von Plasmotyp und Genotyp. Dissertation, Univ. Hohenheim, Stuttgart.
Google Scholar
Scoles G. J., Evans L. E. 1979. The genetics of fertility restoration in cytoplasmic male-sterile rye. Can. J. Genet. Cytol. 21: 417 — 422.
DOI: https://doi.org/10.1139/g79-045
Google Scholar
Authors
Irena Kolasińskai.kolasinska@ihar.edu.pl
Zakład Genetyki i Hodowli Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Statistics
Abstract views: 5PDF downloads: 3
License
Copyright (c) 2025 Irena Kolasińska
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Irena Kolasińska, Jacek Jagodziński, Waldemar Brukwiński, Katarzyna Banaszak, Barbara Kozber, Renata Krysztofik, Michał Materka, Valuable parental components for creation of rye hybrids , Bulletin of Plant Breeding and Acclimatization Institute: No. 278 (2015): Regular issue
- Irena Kolasińska, Results of the breeding program aimed at improvement of male fertility of hybrid rye varieties. , Bulletin of Plant Breeding and Acclimatization Institute: No. 284 (2018): Regular issue
- Irena Kolasińska, Identification of male fertility restorers for the Pampa cytoplasm among inbred lines of rye with different origins , Bulletin of Plant Breeding and Acclimatization Institute: No. 260/261 (2011): Regular issue
- Irena Kolasińska, Waldemar Brukwiński, Jacek Jagodziński, Barbara Kozber, Renata Krzysztofik, Michał Materka, Estimation of combining ability of female components for rye hybrids creation , Bulletin of Plant Breeding and Acclimatization Institute: No. 265 (2012): Regular issue
- Irena Kolasińska, Jacek Jagodziński, Waldemar Brukwiński, Katarzyna Banaszak, Renata Krysztofik, Michał Materka, Identification of valuable parental components for creation of rye hybrids , Bulletin of Plant Breeding and Acclimatization Institute: No. 268 (2013): Regular issue
- Irena Kolasińska, Waldemar Brukwiński, Jacek Jagodziński, Michał Materka, Combining ability of parental genotypes of winter rye , Bulletin of Plant Breeding and Acclimatization Institute: No. 260/261 (2011): Regular issue
- Irena Kolasińska, Progress in the breeding program of the male components of rye hybrids for fertility restoration in the Pampa cytoplasm , Bulletin of Plant Breeding and Acclimatization Institute: No. 281 (2017): Regular issue
- Irena Kolasińska, Identification of gene donors for male fertility restoration in rye hybrids with Pampa cytoplasm , Bulletin of Plant Breeding and Acclimatization Institute: No. 271 (2014): Regular issue
- Andrzej Rafalski, Magdalena Żurawska, Irena Kolasińska, Iwona Wiśniewska, The molecular analysis of male-sterile, maintainer and restorer lines of rye , Bulletin of Plant Breeding and Acclimatization Institute: No. 235 (2005): Regular issue
- Irena Kolasińska, Diversity in fertility restoration among male sterile rye lines with Pampa cytoplasm , Bulletin of Plant Breeding and Acclimatization Institute: No. 264 (2012): Regular issue
