Resistance of winter wheat lines to Fusarium head blight and Fusarium toxins accumulation characterized using different types of resistance
Tomasz Góral
t.goral@ihar.edu.plZakład Fitopatologii, Instytut Hodowli i Aklimatyzacji Roślin — PIB, Radzików (Poland)
Piotr Ochodzki
Zakład Fitopatologii, Instytut Hodowli i Aklimatyzacji Roślin — PIB, Radzików (Poland)
Dorota Walentyn-Góral
Zakład Fitopatologii, Instytut Hodowli i Aklimatyzacji Roślin — PIB, Radzików (Poland)
Jolanta Belter
Zakład Genomiki, Instytut Genetyki Roślin PAN, Poznań (Poland)
Maciej Majka
Zakład Genomiki, Instytut Genetyki Roślin PAN, Poznań (Poland)
Michał Kwiatek
Zakład Genomiki, Instytut Genetyki Roślin PAN, Poznań (Poland)
Halina Wiśniewska
Zakład Genomiki, Instytut Genetyki Roślin PAN, Poznań (Poland)
Jerzy Bogacki
Danko Hodowla Roślin Sp. z o.o. (Poland)
Tdeusz Drzazga
Małopolska Hodowla Roślin HBP, Kraków (Poland)
Bogusława Ługowska
Danko Hodowla Roślin Sp. z o.o. (Poland)
Przemysław Matysik
Hodowla Roślin Strzelce Grupa IHAR (Poland)
Edward Witkowski
Hodowla Roślin Smolice Grupa IHAR (Poland)
Krzysztof Rubrucki
Poznańska Hodowla Roślin Sp. z o.o. (Poland)
Urszula Woźna-Pawlak
Poznańska Hodowla Roślin Sp. z o.o. (Poland)
Abstract
The resistance to Fusarium head blight (FHB) of 224 lines and 10 check cultivars/lines of winter wheat was evaluated in the field experiments in Radzików and Poznań/Cerekwica. These lines originated from breeding programs of winter wheat and from the collection established based on the results of studies on the resistance to FHB in the years 2008-2013. Spikes of wheat were inoculated with Fusarium culmorum. Spike infection and Fusarium kernel damage were scored. In grain of selected 61 lines accumulation of ergosterol, trichothecenes of group B, and zearalenone was analyzed. Wheat genotypes varied significantly in resistance to FHB. The average FHB index amounted to 23.7%. A significant effect of plant height on the FHB index value (r = -0.528) was found. The tested lines showed varied resistance to Fusarium kernel damage (on average:48.4%) and ergosterol content in grain (on average: 30.9 mg·kg-1). The severity of spike infection significantly correlated with Fusarium kernel damage (r = 0.768). Wheat genotypes showed varied resistance to accumulation of Fusarium toxins. Average contents of deoxynivalenol (+ acetyl derivatives) amounted to 20.515 mg·kg-1, while zearalenone to 932 g·kg-1. The spike infection, resistance of type II and Fusarium kernel damage correlated significantly with the contents of ergosterol, deoxynivalenol and zearalenone in the grain. Genotypes integrating the various types of resistance to FHB were identified.
Supporting Agencies
Keywords:
Fusarium culmorum, Fusarium head blight, trichothecenes, wheat, zearalenoneReferences
Argyris J., Sanford D., Van, TeKrony D. 2003. Fusarium graminearum infection during wheat seed development and its effect on seed quality. Crop Sci. 43: 1782 — 1788.
Google Scholar
Bai G., Shaner G. 2004. Management and resistance in wheat and barley to Fusarium head blight. Annu. Rev. Phytopathol. 42: 135 — 161.
Google Scholar
Berthiller F., Dall’Asta C., Schuhmacher R., Lemmens M., Adam G., Krska R. 2005. Masked mycotoxins: determination of a deoxynivalenol glucoside in artificially and naturally contaminated wheat by liquid chromatography-tandem mass spectrometry. J. Agric. Food Chem. 53: 3421 — 3425.
Google Scholar
Bottalico A. 1998. Fusarium diseases of cereals: Species complex and related mycotoxin profiles, in Europe. J. Plant Pathol. 80: 85 — 103.
Google Scholar
Bottalico A., Perrone G. 2002. Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe. Eur. J. Plant Pathol. 108: 611 — 624.
Google Scholar
Boutigny A.-L.L., Richard-Forget F., Barreau C. 2008. Natural mechanisms for cereal resistance to the accumulation of Fusarium trichothecenes. Eur. J. Plant Pathol. 121: 411 — 423.
Google Scholar
Buerstmayr H., Ban T., Anderson J. A. 2009. QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: A review. Plant Breed. 128: 1 — 26.
Google Scholar
Buerstmayr H., Lemmens M., Berlakovich S., Ruckenbauer P. 1999. Combining ability of resistance to head blight caused by Fusarium culmorum (W.G. Smith) in the F1 of a seven parent diallel of winter wheat (Triticum aestivum L.). Euphytica 110: 199 — 206.
Google Scholar
Buerstmayr H., Lemmens M., Schmolke M., Zimmermann G., Hartl L., Mascher F., Trottet M., Gosman N.E., Nicholson P. 2008. Multi-environment evaluation of level and stability of FHB resistance among parental lines and selected offspring derived from several European winter wheat mapping populations. Plant Breed. 127: 325 — 332.
Google Scholar
Draeger R., Gosman N., Steed A., Chandler E., Thomsett M., Srinivasachary, Schondelmaier J., Buerstmayr H., Lemmens M., Schmolke M., Mesterhazy A., Nicholson P. 2007. Identification of QTLs for resistance to Fusarium head blight, DON accumulation and associated traits in the winter wheat variety Arina. Theor. Appl. Genet. 115: 617 — 625.
Google Scholar
Foroud N. A., Eudes, F. 2009. Trichothecenes in cereal grains. Int. J. Mol. Sci. 10: 147 — 173.
Google Scholar
Góral T., Walentyn-Góral D., Wiśniewska H. 2015. Odporność pszenicy i pszenżyta na fuzariozę kłosów — odporność typu I oraz typu II. Biul. IHAR (w druku).
Google Scholar
Góral T., Wiśniewska H., Ochodzki P., Walentyn-Góral, D., Beletr J., Kwiatek M., Bogacki J., Drzazga T., Ługowska B., Matysik P., Witkowski E., Rubrycki K., Woźna-Pawlak U. 2014. Screening of winter wheat breeding lines for resistance to Fusarium head blight and accumulation of Fusarium toxins in grain. Book of Abstracts. Cereals for Food, Feed and Fuel — Challenge for Global Improvement; Joint EUCARPIA Cereal Section & I•T•M•I Conference, 29.06 – 04.07, 2014; Wernigerode, Niemcy: 254 p.
Google Scholar
Gunnaiah R., Kushalappa A.C., Duggavathi R., Fox S., Somers D. J. 2012. Integrated metabolo-proteomic approach to decipher the mechanisms by which wheat QTL (Fhb1) contributes to resistance against Fusarium graminearum. PLoS One. 7: e40695.
Google Scholar
Kollers S., Rodemann B., Ling J., Korzun V., Ebmeyer E., Argillier O., Hinze M., Plieske J., Kulosa D., Ganal M. W., Röder M. S. 2013. Whole genome association mapping of Fusarium head blight resistance in European winter wheat (Triticum aestivum L.). PLoS One. 8: e57500.
Google Scholar
Lemmens M., Koutnik A., Steiner B., Buerstmayr H., Berthiller F., Schuhmacher R., Maier F., Schäfer W. 2008. Investigations on the ability of Fhb1 to protect wheat against nivalenol and deoxynivalenol. Cereal Res. Commun. 36: 429 — 435.
Google Scholar
Mesterházy Á . 2002. Role of deoxynivalenol in aggressiveness of Fusarium graminearum and F. culmorum and in resistance to Fusarium head blight. Eur. J. Plant Pathol. 108: 675 — 684..
Google Scholar
Mesterházy Á. 1995. Types and components of resistance to Fusarium head blight of wheat. Plant Breed. 114: 377 — 386.
Google Scholar
Miedaner T., Reinbrecht C., Lauber U., Schollenberger M., Geiger H. H. 2001. Effects of genotype and genotype-environment interaction on deoxynivalenol accumulation and resistance to Fusarium head blight in rye, triticale, and wheat. Plant Breed. 120:97 — 105.
Google Scholar
Miedaner T., Voss, H.-H. 2008. Effect of dwarfing genes on Fusarium head blight resistance in two sets of near-isogenic lines of wheat and check cultivars. Crop Sci. 48: 2115.
Google Scholar
Miller J. D. 2008. Mycotoxins in small grains and maize: old problems, new challenges. Food Addit Contam. 25: 219 — 30.
Google Scholar
Ochodzki P., Góral T. 2006. Production of mycotoxins by selected Fusarium graminearum and F. culmorum isolates cultured on rice and wheat. Conference Papers of 28. Mykotoxin-Workshop, Bydgoszcz, Poland, 29-31 May 2006: p. 73.
Google Scholar
Paillard S., Schnurbusch T., Tiwari R., Messmer M., Winzeler M., Keller B., Schachermayr G. 2004. QTL analysis of resistance to Fusarium head blight in Swiss winter wheat (Triticum aestivum L.). Theor. Appl. Genet. 109: 323 — 332.
Google Scholar
Perkowski J., Buśko M., Stuper K., Kostecki M., Matysiak A., Szwajkowska-Michałek L. 2008. Concentration of ergosterol in small-grained naturally contaminated and inoculated cereals. Biologia 63: 542 — 547.
Google Scholar
Perkowski J., Miedaner T., Geiger H. H., Muller H. M., Chelkowski J. 1995. Occurrence of deoxynivalenol (DON), 3-acetyl-DON, zearalenone, and ergosterol in winter rye inoculated with Fusarium culmorum. Cereal Chem. 72: 205 — 209.
Google Scholar
Perkowski J., Wiwart M., Busko M., Laskowska M., Berthiller F., Kandler W., Krska R. 2007. Fusarium toxins and total fungal biomass indicators in naturally contaminated wheat samples from north-eastern Poland in 2003. Food Addit Contam. 24: 1292 — 1298.
Google Scholar
Semagn K., Skinnes H., Bjørnstad Å., Marøy A.G., Tarkegne Y. 2007. Quantitative trait loci controlling Fusarium head blight resistance and low deoxynivalenol content in hexaploid wheat population from ‘Arina’ and NK93604. Crop Sci. 47: 294.
Google Scholar
Snijders C. H. A. 1990. Fusarium head blight and mycotoxin contamination of wheat, a review. Netherlands J Plant Pathol. 96: 187 — 198.
Google Scholar
Srinivasachary, Gosman N., Steed A., Simmonds J., Leverington-Waite M., Wang Y., Snape J., Nicholson P. 2008. Susceptibility to Fusarium head blight is associated with the Rht-D1b semi-dwarfing allele in wheat. Theor. Appl. Genet. 116: 1145 — 1153.
Google Scholar
Wiśniewska H., Góral T., Ochodzki P., Walentyn-Góral D., Kwiatek M., Majka M., Grzeszczak I., Beletr J., Banaszak Z., Pojmaj M., Kurleto D., Konieczny M., Budzianowski G., Cicha A., Paizert K., Woś H. 2014. Odporność rodów hodowlanych pszenżyta ozimego na fuzariozę kłosów. Biul. IHAR 271: 29 — 43.
Google Scholar
Wiśniewska H., Kowalczyk, K. 2005. Resistance of cultivars and breeding lines of spring wheat to Fusarium culmorum and powdery mildew. J. Appl. Genet. 46: 35 — 40.
Google Scholar
Yan W., Li, H. B., Cai S.B., Ma H.X., Rebetzke, G.J., Liu C. J. 2011. Effects of plant height on type I and type II resistance to Fusarium head blight in wheat. Plant Pathol. 60: 506 — 512.
Google Scholar
Zhu H., Gilchrist L., Hayes P., Kleinhofs A., Kudrna D., Liu Z., Prom L., Steffenson B., Toojinda T., Vivar H. 1999. Does function follow form? Principal QTLs for Fusarium head blight (FHB) resistance are coincident with QTLs for inflorescence traits and plant height in a doubled-haploid population of barley. Theor. Appl. Genet. 99: 1221 — 1232.
Google Scholar
Authors
Tomasz Góralt.goral@ihar.edu.pl
Zakład Fitopatologii, Instytut Hodowli i Aklimatyzacji Roślin — PIB, Radzików Poland
Authors
Piotr OchodzkiZakład Fitopatologii, Instytut Hodowli i Aklimatyzacji Roślin — PIB, Radzików Poland
Authors
Dorota Walentyn-GóralZakład Fitopatologii, Instytut Hodowli i Aklimatyzacji Roślin — PIB, Radzików Poland
Authors
Jolanta BelterZakład Genomiki, Instytut Genetyki Roślin PAN, Poznań Poland
Authors
Maciej MajkaZakład Genomiki, Instytut Genetyki Roślin PAN, Poznań Poland
Authors
Michał KwiatekZakład Genomiki, Instytut Genetyki Roślin PAN, Poznań Poland
Authors
Halina WiśniewskaZakład Genomiki, Instytut Genetyki Roślin PAN, Poznań Poland
Authors
Jerzy BogackiDanko Hodowla Roślin Sp. z o.o. Poland
Authors
Tdeusz DrzazgaMałopolska Hodowla Roślin HBP, Kraków Poland
Authors
Bogusława ŁugowskaDanko Hodowla Roślin Sp. z o.o. Poland
Authors
Przemysław MatysikHodowla Roślin Strzelce Grupa IHAR Poland
Authors
Edward WitkowskiHodowla Roślin Smolice Grupa IHAR Poland
Authors
Krzysztof RubruckiPoznańska Hodowla Roślin Sp. z o.o. Poland
Authors
Urszula Woźna-PawlakPoznańska Hodowla Roślin Sp. z o.o. Poland
Statistics
Abstract views: 83PDF downloads: 51
License
Copyright (c) 2015 Tomasz Góral, Piotr Ochodzki, Dorota Walentyn-Góral, Jolanta Belter, Maciej Majka, Michał Kwiatek, Halina Wiśniewska, Jerzy Bogacki, Tdeusz Drzazga, Bogusława Ługowska, Przemysław Matysik, Edward Witkowski, Krzysztof Rubrucki, Urszula Woźna-Pawlak
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Dorota Jasińska, Anetta Kuczyńska, Krzysztof Mikołajczak, Piotr Ogrodowicz, Hanna Ćwiek-Kupczyńska, Magdalena Anioła, Krzysztof Rubrycki, Róża Mazur, Michał Kempa, Maria Surma, Tadeusz Adamowski, Halina Wiśniewska, Variation of spring barley SSD lines in reaction to infection by fungi of the genus Fusarium , Bulletin of Plant Breeding and Acclimatization Institute: No. 282 (2017): Regular issue
- Krystyna Witkowska, Edward Witkowski, Tadeusz Śmiałowski, Ada Bogusławska, Dominik Dwojak, Effect of frost resistance and winter hardiness on winter wheat breeding lines yielding in the season 2015/2016 , Bulletin of Plant Breeding and Acclimatization Institute: No. 281 (2017): Regular issue
- Kinga Stuper-Szablewska, Anna Ostrowska, Tomasz Góral, Anna Matysiak, Juliusz Perkowski, Comparison of the antioxidant activity of different varieties of winter wheat naturally infected and inoculated with fungi of the genus Fusarium , Bulletin of Plant Breeding and Acclimatization Institute: No. 276 (2015): Regular issue
- Dariusz R. Mańkowski, Michał Kwiatek, Introduction to Issue 300 of the Bulletin of Plant Breeding and Acclimatization Institute , Bulletin of Plant Breeding and Acclimatization Institute: No. 300 (2023): Regular issue
- Krystyna Witkowska, Tadeusz Śmiałowski, Edward Witkowski, Dependence of yield of winter wheat strains on the degree of infection by Stagonospora nodorum and Puccinia triticina in different conditions of field experiments , Bulletin of Plant Breeding and Acclimatization Institute: No. 262 (2011): Regular issue
- Halina Wiśniewska, Tomasz Góral, Piotr Ochodzki, Dorota Walentyn-Góral, Michał Kwiatek, Maciej Majka, Iga Grzeszczak, Jolanta Belter, Zofia Banaszak, Mirosław Pojmaj, Danuta Kurleto, Marcin Konieczny, Grzegorz Budzianowski, Alicja Cicha, Kazimierz Paizert, Henryk Woś, Resistance of winter triticale breeding lines to Fusarium head blight , Bulletin of Plant Breeding and Acclimatization Institute: No. 271 (2014): Regular issue
- Krystyna Witkowska, Edward Witkowski, Tadeusz Śmiałowski, Jacek Waga, Relationship between wheat storage proteins and winter hardiness in chosen hybrids of winter wheat , Bulletin of Plant Breeding and Acclimatization Institute: No. 268 (2013): Regular issue
- Edward Witkowski, Krystyna Witkowska, Urszula Woźna-Pawlak, Krzysztof Rubrycki, Przemysław Matysik, Maria Bogacka, Helena Bielerzewska, Marcin Konieczny, Małgorzata Łagodzka-Gola, Tadeusz Drzazga, Tadeusz Śmiałowski, Usefulness of frost resistance assessments for prediction of winter hardiness of winter wheat cultivars , Bulletin of Plant Breeding and Acclimatization Institute: No. 268 (2013): Regular issue
- Tomasz Góral, Kinga Stuper-Szablewska, Anna Przybylska, Anna Matysik, Juliusz Perkowski, The influence of inoculation with fungi of the genus Fusarium on beta-sitosterol content in grain of different varieties of winter wheat , Bulletin of Plant Breeding and Acclimatization Institute: No. 281 (2017): Regular issue
- Tomasz Góral, Dorota Walentyn-Góral, Resistance of spring wheat cultivars and lines to Fusarium head blight caused by Fusarium culmorum , Bulletin of Plant Breeding and Acclimatization Institute: No. 271 (2014): Regular issue