Obtaining of haploids with use of Hordeum bulbosum method and mutagenesis and analysis of quantitative traits variation in spring barley DH lines
Wojciech Rybiński
office@igr.poznan.plInstytut Genetyki Roślin PAN w Poznaniu (Poland)
Tadeusz Adamski
Instytut Genetyki Roślin PAN w Poznaniu (Poland)
Maria Surma
Instytut Genetyki Roślin PAN w Poznaniu (Poland)
Jan Bocianowski
Uniwersytet Przyrodniczy w Poznaniu (Poland)
Abstract
In performed studies on the use of mutagenesis in DH system, the seeds of malting barley cultivar Rudzik were treated with laser light and chemomutagen — N-methyl-N-nitroso urea (MNU). The plants grown from the treated plants were crossed with Hordeum bulbosum. The effectiveness of haploid production was measured by proportion of the obtained seeds to the number of pollinated florets and number of haploids to number of pollinated florets and immature embryos transferred to in vitro culture. The obtained results as compared to control (non-treated seeds) indicates that the applied dose of MNU decreased the effectiveness of haploid production. In result of diploidization, a numerous DH plants were obtained which were propagated and used for estimation of quantitative traits variability in a field trial. The use of multivariate statistical method indicates that DH lines were characterized by a broader range of traits variability in comparison with their initial cultivar Rudzik.
Keywords:
dihaploid lines, Hordeum bulbosum, mutagen, spring barley, quantitative traitsReferences
Adamski T., Jeżowski S., Majewska T., Surma M. 1990. Efektywność metody bulbosowej w zależności od genotypu Hordeum vulgare i Hordeum bulbosum. Hodowla Roślin 4/ 5: 1 — 5.
Google Scholar
Beversdorf W.D., Kott L.S. 1987. An in vitro mutagenesis/selection system for Brassica napus. Iowa State J. Res. 61: 435 — 443.
Google Scholar
Caliński T., Dyczkowska A., Kaczmarek Z. 1976. Algorytmy Biometrii i Statystyki. Zeszyt 5: 77 — 113.
Google Scholar
Ceranka B., Chudzik H., Czajka S., Kaczmarek Z. 1997. Wieloimienna analiza wariancji dla doświadczeń wieloczynnikowych. Algorytmy Biometrii i Statystyki. Zeszyt 6: 51 — 60.
Google Scholar
Devaux P., Adamski T., Surma M. 1990. Studies on low cross abilities encountered with the Hordeum bulbosum method for haploid production of barley, Hordeum vulgare L. Plant Breeding 104: 305 — 311.
Google Scholar
Ehrenberg L., Gustafsson A., Lundqvist U. 1961. Viable mutants induced in barley by ionizing radiation and chemical mutagens. Hereditas 47: 243 — 282.
Google Scholar
Kapała A., Rybiński W. 1996. Zmienność i jakość białka oraz zdolność plonowania u krótkosłomych mutantów jęczmienia jarego. Biul. IHAR 200: 191 — 195.
Google Scholar
Kasha K.J., Kao K.N. 1970. High frequency haploid production in barley (Hordeum vulgare L.). Nature 225: 874 — 876.
Google Scholar
Kirishnaswami R. 1967. Radiation induced dwarf mutants in barley. Curr. Sci. 36(12): 327 — 328.
Google Scholar
Kozak M., Bocianowski J., Sawkojć S., Wnuk A. 2010. Call for more graphical elements in statistical teaching and consultancy. Biometrical Letters 47(1): 57 — 68.
Google Scholar
Kozak M., Bocianowski J., Rybiński W. 2013. Note on the use of variation for data from agricultural factorial experiments. Bulgarian J. Agric. Sci. 19 (4): 644 — 646.
Google Scholar
Kumar S., Bansal H. C., Singh D., Swaminathan M. S. 1967. Pathways of height reduction in induced dwarf mutation in barley. Z. Pflanzenzüchtung 57: 317 — 324.
Google Scholar
Laib Y., Szarejko I., Polok K., Małuszyński M. 1996. Barley anther culture for doubled haploid mutant production. Mutation Breeding Newsletter 42: 13 — 15.
Google Scholar
Małuszyński M., Małuszyńska J., Malepszy S. 1978. Wybrane zagadnienia mutagenezy roślin wyższych. Mutagenza Roślin Wyższych. Materiały III ogólnopolskiej konferencji mutacyjnej. Uniwersytet Śląski, Katowice 1978: 15 — 30.
Google Scholar
Pickering R.A., Devaux P. 1992. Haploid production: Approaches and use in plant breeding. In: Barley Genetics, Biochemistry, Molecular Biology and Biotechnology (P.R. Shewry ed.). C.A.B. Int. Wallinford, UK: 519 — 547.
Google Scholar
Rencher A.C. 1998. Interpretation of canonical discriminant functions, canonical variates, and principal components. Am. Stat. 46: 217 — 225.
Google Scholar
Rybiński W. 1981. Short-straw forms of spring barley (Hordeum vulgare L.) obtained as a result of MNUA- induced mutations. Genetica Polonica 22 (3): 271 — 287.
Google Scholar
Rybiński W. 1998. Wykorzystanie światła lasera w badaniach nad roślinami wyższymi. Zastosowanie kultur in vitro w fizjologii roślin (F. Dubert, M. Rapacz – edytorzy), Kraków 1998: 169 — 178.
Google Scholar
Rybiński W., Patyna H., Przewoźny T. 1993. Mutagenic effect of laser and chemical mutagens in barley (Hordeum vulgare L.). Genetica Polonica 34 (3): 337 — 343.
Google Scholar
Rybiński W., Adamski T., Surma M. 2000. Wykorzystanie światła lasera i chemomutagenu w badaniach nad uzyskaniem linii podwojonych haploidów jęczmienia. Zesz.ty Probl. Post. Nauk Roln. 473: 291 — 298.
Google Scholar
Rybiński W. 2001. Influence of laser beams combined with chemomutagen (MNU) on the variability of traits and mutation frequency in spring barley. Int. Agrophysics 15: 115 — 119.
Google Scholar
Rybiński w. 2003. Mutagenesis as a tool for improvement of traits in grasspea (Lathyrus sativus L.). Edited by Colin Hanbury, CLIMA, Australia, Lathyrus Lathyrism Newsletter 3: 27 — 31.
Google Scholar
Rybiński W., Pietruszewski S., Kornarzyński K. 2003. Influence of magnetic field with chemomutagen and gamma rays on the variability of yielding parameters in barley. Int. Agrophysics 17: 85 — 91.
Google Scholar
Rybiński W., Pankiewicz K., Bocianowski J., Rębarz M. 2008.Analiza wybranych cech na poziomie fenotypowym i molekularnym u dwu- i wielorzędowych linii podwojonych haploidów jęczmienia jarego (Hordeum vulgare L.) Biul. IHAR 249: 141 — 155.
Google Scholar
Surma M. 1997. Wykorzystanie zjawiska eliminacji chromosomów w hodowli jęczmienia. Akademia Rolnicza w Poznaniu. Seminaria Wydziału Rolniczego, Zeszyt 3: 157 — 174.
Google Scholar
Swanson E. B., Herrgesell M. J., Arnold M., Sippel D. W., Wong R. S. C. 1989. Microspore mutagenesis and selection: Canola plant with field tolerance to the imidazolinones. Theor. Appl. Genet. 78: 525 — 530.
Google Scholar
Szarejko I., Małuszyński M., Polok K., Kilian A. 1991. Plant Mutation Breeding for Crop Improvement. Proceedings of a Symposium, Vienna, 18–22 June 1990: 355 — 378.
Google Scholar
Umba Di-Umba, Małuszyński M., Szarejko I., Zbieszczyk J. 1991. High frequency of DH-mutants from M1 after mutagenic treatment with MNH and sodium azide. Mutation Breeding Newsletter 38: 8 — 9.
Google Scholar
Zapata F. J., Aldemita R. R. 1989. „Induction of salt tolerance in high yielding rice varieties through mutagenesis and anther culture”, Current Options for Cereal Improvement (Małuszyński M., ed.). Kluwer Academic Publishers, Dordrecht: 193 — 202.
Google Scholar
Authors
Tadeusz AdamskiInstytut Genetyki Roślin PAN w Poznaniu Poland
Authors
Maria SurmaInstytut Genetyki Roślin PAN w Poznaniu Poland
Authors
Jan BocianowskiUniwersytet Przyrodniczy w Poznaniu Poland
Statistics
Abstract views: 266PDF downloads: 36
License
Copyright (c) 2015 Wojciech Rybiński, Tadeusz Adamski, Maria Surma, Jan Bocianowski
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Joanna Wolko, Agnieszka Dobrzycka, Jan Bocianowski, Estimation of the seed yield traits heterosis for single cross and three-way cross hybrids of oilseed rape (Brassica napus L.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 287 (2019): Special issue
- Jan Bocianowski, Henryk Brzeskwiniewicz, Tadeusz Łuczkiewicz, Influence of testers selection on estimation of GCA effects in spring oilseed rape inbred lines , Bulletin of Plant Breeding and Acclimatization Institute: No. 250 (2008): Regular issue
- Katarzyna Ambroży, Ewa Bakinowska, Jan Bocianowski, Anna Budka, Wiesław Pilarczyk, Bogna Zawieja, Statistical support of selection decisions at early stage of cereal breeding. Part II. Empirical comparison of treatment effects estimation methods , Bulletin of Plant Breeding and Acclimatization Institute: No. 250 (2008): Regular issue
- Wojciech Rybiński, Jan Bocianowski, Katarzyna Pankiewicz, Variability of morphological and yield-contributing traits of grasspea (Lathyrus sativus L.) mutants , Bulletin of Plant Breeding and Acclimatization Institute: No. 249 (2008): Regular issue
- Katarzyna Ambroży, Ewa Bakinowska, Jan Bocianowski, Anna Budka, Wiesław Pilarczyk, Bogna Zawieja, Statistical support of selection decisions at early stage of cereal breeding. Part I. Methods of estimation of treatment effects , Bulletin of Plant Breeding and Acclimatization Institute: No. 250 (2008): Regular issue
- Tadeusz Adamski, Zygmunt Kaczmarek, Maria Surma, Anetta Kuczyńska, Karolina Krystkowiak, Bolesław Salmanowicz, Renata Trzeciak, Zofia Banaszak, Bogusława Ługowska, Małgorzata Majcher, Wiktor Obuchowski, Multivariate analysis of some quality traits of winter wheat grain , Bulletin of Plant Breeding and Acclimatization Institute: No. 260/261 (2011): Regular issue
- Maria Surma, Tadeusz Adamski, Anetta Kuczyńska, Karolina Krystkowiak, Renata Trzeciak, Krzysztof Mikołajczak, Piotr Ogrodowicz, Modified single seed descent technique in winter barley breeding , Bulletin of Plant Breeding and Acclimatization Institute: No. 262 (2011): Regular issue
- Tadeusz Adamski, Andrzej Bichoński, Zdzisław Biliński, Zbigniew Bystry, Piotr Jarosz, Dorota Jasińska, Zygmunt Kaczmarek, Karolina Krystkowiak, Anetta Kuczyńska, Wojciech Mikulski, Barbara Nowak, Wanda Orłowska-Job, Zdzisław Paszkiewicz, Michał Rębarz, Maria Surma, Anna Sybilska, Renata Trzeciak, The genotype-environment interaction for barley lines from different breeding stations , Bulletin of Plant Breeding and Acclimatization Institute: No. 249 (2008): Regular issue
- Agnieszka Dobrzycka, Joanna Wolko, Jan Bocianowski, Kamila Nowosad, The characteristics of phenotypic variation in DH lines and hybrids of winter oilseed rape (Brassica napus L.) including yield related traits , Bulletin of Plant Breeding and Acclimatization Institute: No. 287 (2019): Special issue
- Anita Dobek, Jan Bocianowski, Ewa Bakinowska, Wiesław Pilarczyk, Wojciech Mikulski, Jacek Kaczmarek, Problems related to testing of cereal lines at early stages of plant breeding , Bulletin of Plant Breeding and Acclimatization Institute: No. 249 (2008): Regular issue
