Identification of gene donors for male fertility restoration in rye hybrids with Pampa cytoplasm
Irena Kolasińska
i.kolasinska@ihar.edu.plZakład Genetyki i Hodowli Roślin, Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy w Radzikowie (Poland)
Abstract
The objective of this study was to identify effective male fertility restorers among rye genotypes developed from Polish varieties, Turkish landrace populations and specially created breeding populations. Restoring ability of numerous inbred lines developed from these groups of rye populations were studied in the years 2009–2013. Single plants of inbred lines were crossed to a hard-restoration tester (CMS-Tt) under isolation bags. The level of male fertility of test hybrids was assessed in plastic tunnels and/or in field by scoring visually pollen shedding of plant plots and/or anther dehiscence of single plants in a 1–9 scale. The restoration indices (RI) were calculated according to the formula: RI = % of male fertile (% mf) plants + 1/2% of partially male fertile plants (% pmf). Low frequency (mean 3%) of effective restorers (RI above 70%) was found among the inbred lines derived from Polish varieties. Majority of inbred lines of this group were only partial restorers. In contrast, the inbred lines developed from specially created breeding populations were highly effective in restoration of male fertility of their test hybrids (68%). Moreover, inbred lines derived from the Turkish landrace populations proved to be very effective restorers. Frequency of effective restorers among genotypes assessed in 2009 and 2010 was 48.3% and 82.3%, respectively. Several genotypes with different origins were able to restore completely pollen fertility of their test hybrids cultivated in plastic tunnels and in field conditions (IR = 100%; % mf = 100). Introduction of these restoring gene donors into the father pool should result in improved male fertility in Pampa-based rye hybrids. Utilization of identified restoring gene donors in breeding programe of rye hybrids was discussed.
Keywords:
hybrid breeding, male sterility, Pampa cytoplasm, restorer identification, restoration of male fertility, ryeReferences
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Authors
Irena Kolasińskai.kolasinska@ihar.edu.pl
Zakład Genetyki i Hodowli Roślin, Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy w Radzikowie Poland
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