Relationship between wheat storage proteins and winter hardiness in chosen hybrids of winter wheat
Krystyna Witkowska
smolice@hrsmolice.plHodowla Roślin Smolice Sp. z o.o. Grupa IHAR (Poland)
Edward Witkowski
Hodowla Roślin Smolice Sp. z o.o. Grupa IHAR (Poland)
Tadeusz Śmiałowski
Zakład Roślin Zbożowych IHAR — PIB w Krakowie (Poland)
Jacek Waga
Zakład Roślin Zbożowych IHAR — PIB w Krakowie (Poland)
Abstract
The aim of these studies was to find out whether polymorphic wheat storage proteins (gliadins and HMW glutenins) and winter hardiness are interrelated. Two sets of winter wheats were analyzed. In the first set 19 groups of hybrid genotypes (90 lines in total) of F5 generation from cross combinations between common wheat (Triticum aestivum ssp. vulgare) cultivars and strains were analyzed. The second set comprised four pairs of hybrid genotypes originating from crossing between spelt (Triticum aestivum ssp. spelta, cv. Obercummer Rotkorn) and common wheat breeding strain LAD 480. Gliadins and HMW glutenins extracted from individual kernels of analyzed hybrid genotypes were separated using electrophoretic techniques: A-PAGE and SDS-PAGE. The obtained results suggested close relationships between HMW glutenin subunits: Glu A1-1, Glu B1-7+9/6+8, Glu D1-5+10 and gliadin null alleles localized on the chromosome 6B (locus Gli B2) and winter hardiness of winter wheat.
Supporting Agencies
Keywords:
electrophoresis, bichemical markers, winter wheat, winter hardinessReferences
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Authors
Edward WitkowskiHodowla Roślin Smolice Sp. z o.o. Grupa IHAR Poland
Authors
Tadeusz ŚmiałowskiZakład Roślin Zbożowych IHAR — PIB w Krakowie Poland
Authors
Jacek WagaZakład Roślin Zbożowych IHAR — PIB w Krakowie Poland
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Copyright (c) 2013 Krystyna Witkowska, Edward Witkowski, Tadeusz Śmiałowski, Jacek Waga
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