Initiation of in vitro cultures and micropropagation of sida (Sida hermaphrodita R.)
Grażyna Mańkowska
sekretariat@iwnirz.plZakład Biotechnologii, Instytutu Włókien Naturalnych i Roślin Zielarskich w Poznaniu (Poland)
Aleksandra Luwańska
Zakład Biotechnologii, Instytutu Włókien Naturalnych i Roślin Zielarskich w Poznaniu (Poland)
Karolina Grygorowicz
Zakład Biotechnologii, Instytutu Włókien Naturalnych i Roślin Zielarskich w Poznaniu (Poland)
Karolina Wielgus
Zakład Biotechnologii, Instytutu Włókien Naturalnych i Roślin Zielarskich w Poznaniu (Poland)
Abstract
Sida (Sida hermaphrodita R.) can be propagated by both generative and vegetative methods by seedlings obtained from root sections, from above ground shoots and from underground part of the plant fragmented into smaller pieces. The problem in generative propagation is low germination rate of seeds caused by hard shell. This causes germination rate in favourable condition to reach only 30– 40%. Imperfection of traditional propagation methods of sida, high price of good quality seeds and growing interest in cultivation of the crop for energy and industrial raw materials caused numerous attempts to propagate the plant by in vitro regeneration cultures. The studies on initiation of micro propagation showed that multiplication factor on MS medium, containing 30 g/l sucrose and 0.01 mg/l BAP was 6.05 for apical explants and for lateral explants 5.25 on the same medium. The MS medium provided a high ratio (97%) of rooted explants ready for acclimatization.
Keywords:
Sida hermaphrodita, in vitro culture, micropropagation, multiplication factorReferences
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Authors
Grażyna Mańkowskasekretariat@iwnirz.pl
Zakład Biotechnologii, Instytutu Włókien Naturalnych i Roślin Zielarskich w Poznaniu Poland
Authors
Aleksandra LuwańskaZakład Biotechnologii, Instytutu Włókien Naturalnych i Roślin Zielarskich w Poznaniu Poland
Authors
Karolina GrygorowiczZakład Biotechnologii, Instytutu Włókien Naturalnych i Roślin Zielarskich w Poznaniu Poland
Authors
Karolina WielgusZakład Biotechnologii, Instytutu Włókien Naturalnych i Roślin Zielarskich w Poznaniu Poland
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Copyright (c) 2013 Grażyna Mańkowska, Aleksandra Luwańska, Karolina Grygorowicz, Karolina Wielgus
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