Modified single seed descent technique in winter barley breeding
Maria Surma
office@igr.poznan.plInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Tadeusz Adamski
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Anetta Kuczyńska
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Karolina Krystkowiak
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Renata Trzeciak
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Krzysztof Mikołajczak
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Piotr Ogrodowicz
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Abstract
Single seed descent technique (SSD) is frequently used in cereal breeding to obtain homozygous lines. It is a modified method of classical ramsh (bulk) and it is based on random choice of one seed from each individual plant in each generation (number of individuals is dependent on assumed number of lines to obtain), starting from F2 hybrids. In F5 or later generation all seeds from each plant are harvested and progeny of a single plant is treated as a SSD line. In the present paper a modification of this technique is presented, which combines the procedure with in vitro culture of immature embryos. The modified SSD technique allows elimination of seed dormancy and acceleration of the breeding process. It was shown that the use of proposed technique in early stages of winter barley breeding permits to accelerate breeding cycle by 2-3 years.
Supporting Agencies
Keywords:
immature embryos, in vitro culture, winter barley, SSD lines, vernalizationReferences
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Authors
Maria Surmaoffice@igr.poznan.pl
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Authors
Tadeusz AdamskiInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Authors
Anetta KuczyńskaInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Authors
Karolina KrystkowiakInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Authors
Renata TrzeciakInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Authors
Krzysztof MikołajczakInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Authors
Piotr OgrodowiczInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
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Copyright (c) 2011 Maria Surma, Tadeusz Adamski, Anetta Kuczyńska, Karolina Krystkowiak, Renata Trzeciak, Krzysztof Mikołajczak, Piotr Ogrodowicz
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