Propagation of valuable genotype of Aesculus hippocastanum L. by micropropagation
Aleksandra Luwańska
sekretariat@iwnirz.plZakład Biotechnologii, Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu (Poland)
Karolina Jarzyniak
Zakład Biotechnologii, Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu (Poland)
Joanna Makowiecka
Zakład Biotechnologii, Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu (Poland)
Karolina Wielgus
Zakład Biotechnologii, Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu (Poland)
Abstract
Horse chestnut (Aesculus hippocastanum L.) is a deciduous tree, native to Balkan Peninsula. It is one of the most popular ornamental trees in Poland and all over the Europe. Thanks to the aescin, A. hippocastanum is widely used for medicinal and cosmetic preparations. The horse chestnut leaf miner moth (Cameraria ohridella) is the most dangerous parasite for European horse chestnut. The insect induces tree defoliation. Second leafing and flowering occur, which result in decrease of the frost hardiness. The most frequently used control measures, although have impact on the leaf miner populations, do not protect the tree completely. The use of modern biotechnology to micropropagate unique genotypes could save this woody plant. The aim of this study was to establish method of efficient propagation protocol for the A. hippocastanum genotype resistant to the horse chestnut life miner using micropropagation. Explants from crown branches were used for culture initiation. Explants were sterilized by rinsing in 15% NaClO and then placed on propagation medium — MS ½ NO3 supplemented with (1 mg/l) BAP. Plant material was transferred to a fresh medium every 4 weeks. The propagation gave satisfying results. The mean number of newly formed shoots was 3–5 during five multiplication cycles. For root induction different treatments were compared. However, all media which were used, did not bring expected result. Therefore, research with rooting and acclimatization of A. hippocastanum needs to be continued.
Keywords:
Aesculus hippocastanum, Cameraria ohridella, in vitro culture, micropropagationReferences
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Authors
Aleksandra Luwańskasekretariat@iwnirz.pl
Zakład Biotechnologii, Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu Poland
Authors
Karolina JarzyniakZakład Biotechnologii, Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu Poland
Authors
Joanna MakowieckaZakład Biotechnologii, Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu Poland
Authors
Karolina WielgusZakład Biotechnologii, Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu Poland
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Copyright (c) 2012 Aleksandra Luwańska, Karolina Jarzyniak, Joanna Makowiecka, Karolina Wielgus
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