The use of molecular markers for introduction of leaf rust (Puccinia recondita f. sp. tritici) and powdery mildew (Blumeria graminis f. sp. tritici) resistance genes in winter wheat (Triticum aestivum)
Aleksandra Pietrusińska
a.pietrusinska@ihar.edu.plPracownia Genetyki Stosowanej, Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików (Poland)
Abstract
The aim of the presented research was to use genetic markers in process of introduction of effective resistance genes to the wheat. A leaf rust resistance gene Lr41 derived from Triticum tauschii (syn. Aegilops squarrosa) and powdery mildew resistance gene Pm21 derived from Dasypyrum villosum (syn. Haynaldia villosa) were introgressed into the best German variety. Line WGRC10 was used as the resistance source of Lr41 for leaf rust, whereas the 6VS/6AL translocation line Yangmai 5 was used as the resistance source of Pm21 for powdery mildew. In foreground selection several molecular markers for Lr41 and Pm21 were applied. For the detection of gene Lr41 in breeding materials with leaf rust resistance five SSR markers: Gdm35, Barc124, Gwm261, Gwm296 and Gwm210 were used. To detect the Pm21 gene three molecular markers: SCAR1250, SCAR1400 and NAU/xibao were development. After the first backcrosses background selection was conducted using the AFLP markers. For the greenhouse screening of the second and the third generations of plants for leaf rust and powdery mildew resistance, plants of selected lines were inoculated at the three-leaf stage with natural pathogen populations of B. graminis and P. recondita. The marker-assisted selection and pathology test allowed to obtain homozygous lines carrying the genes resistance to leaf rust and powdery mildew of winter wheat.
Supporting Agencies
Keywords:
Blumeria graminis, Puccinia recondita, Lr41, Pm21, pyramiding breedingReferences
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Authors
Aleksandra Pietrusińskaa.pietrusinska@ihar.edu.pl
Pracownia Genetyki Stosowanej, Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików Poland
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