Application of RAPD and ISSR methods to genetic similarity estimation of Greek Dasypyrum villosum (L.) P. Candargy populations
Agnieszka Grądzielewska
genetyka.roslin@up.lublin.plInstytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie (Poland)
Edyta Paczos-Grzęda
Instytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie (Poland)
Daniela Gruszecka
Instytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie (Poland)
Abstract
Estimation of genetic similarity between nine Greek Dasypyrum villosum L. (P.) Candargy populations native to three Greece regions (Thesally, West and Central Macedonia) was performed basing on polymorphism of RAPD and ISSR markers. Eighteen RAPD and seventeen ISSR selected primers were used in the genetic similarity analyses. In total, 122 RAPD and 210 ISSR products were obtained, out of which 50% were polymorphic. The RAPD method identified specific products only in five from nine populations studied, while ISSR in seven, most for W6 7264. Polymorphic information content (PIC) values calculated for the both methods, ranged from 0.13–0.68 for RAPD and 0.15–0.52 for ISSR. Mean values of PIC for RAPD and ISSR were 0.32 and 0.33, respectively. Based on the polymorphism of RAPD and ISSR markers and both methods combined, genetic similarities using Dice algorithm were estimated between pairs of populations analyzed. Mean genetic similarities were 0.854, 0.871 and 0.864, respectively. Most different from the others was W6 7264. Based on Dice matrix similarities, dendrograms showing relatedness between the analyzed populations were constructed.
Keywords:
Dasypyrum, ISSR, RAPD, genetic similarity, molecular markersReferences
Bednarek P. T., Chwedorzewska K. 2001. Markery molekularne i ich charakterystyka genetyczna oraz wybrane zastosowania w analizie genetycznej roślin. Biotechnologia 1 (52): 9 — 34.
Google Scholar
Bolibok H., Rakoczy-Trojanowska M., Hromada A., Pietrzykowski R. 2005. Efficiency of different PCR-based marker systems in assessing genetic diversity among winter rye (Secale cereale L.) inbred lines. Euphytica 146: 109 — 116.
Google Scholar
Caceres M.E., De Pace C., Scarascia Mugnozza G.T., Kotsonis P., Ceccarelli M., Cionini P.G. 1998. Genome size variations within Dasypyrum villosum: correlations with chromosomal traits, environmental factors and plant phenotypic characteristics and behaviour in reproduction. Theor. Appl. Genet. 96: 559 — 567.
Google Scholar
Castagana R., Gnocchi S., Perenzin M., Heun M. 1997. Genetic variability of the wild diploid wheat Triticum urartu revealed by RFLP and RAPD markers. Theor. Appl. Genet. 94: 424 — 430.
Google Scholar
Chen Q., Conner R. L., Li H., Laroche A., Graf R.J., Kuzyk A. D. 2002. Expression of resistance to stripe rust, powdery mildew and the wheat curl mite in Triticum aestivum-Haynaldia villosa lines. Can. J. Plant. Sci. 82 (2): 451 — 456.
Google Scholar
De Pace C., Snidaro D., Ciaffi M., Vittori D., Ciofo A., Cenci A., Tanzarella O.A., Qualset C.O., Scarascia Mugnozza G.T. 2001. Introgression of Dasypyrum villosum chromatin into common wheat improves grain protein quality. Euphytica 117: 67 — 75.
Google Scholar
Fahima T., Sun G. L., Beharav A., Krugman T., Beiles A., Nevo E. 1999. RAPD polymorphism of wheat emmer populations, Triticum dicoccoides, in Israel. Theor. Appl. Genet 98: 434 — 447.
Google Scholar
Fernández M. E., Figueiras A. M., Benito C. 2002. The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin. Theor. Appl. Genet. 104: 845 — 851.
Google Scholar
Grądzielewska A. 2006. The genus Dasypyrum — part 2. Dasypyrum villosum — a wild species used in wheat improvement. Euphytica 152: 441 — 454.
Google Scholar
Gruszecka D., Miąc A. 2002. Wykorzystanie metod RAPD w określaniu mieszańcowego charakteru rodów Secale cereale L. × Dasypyrum villosum (L.) P. Candargy. Zeszyty Problemowe Postepów Nauk Rolniczych 488: 161 — 168.
Google Scholar
Matos M., Pinto-Carnide O., Benito C. 2001. Phylogenetic relationship among Portuguese rye based on isozyme, RAPD and ISSR markers. Hereditas 134: 229 — 236.
Google Scholar
Milligan B. G. 1992. Plant DNA isolation. In: Molecular analysis of populations: a practical approach. IRL Press, Oxford, UK, 59 — 88.
Google Scholar
Nagaoka T., Ogihara Y. 1997. Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theor. Appl. Genet. 94: 597 — 602.
Google Scholar
Nei M. 1973. Analysis of gene diversity in subdivided populations. Proc Natl. Acad. Sci. U. S. A. 70, 3321 — 3323.
Google Scholar
Nei M., Li W.H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269 — 5273.
Google Scholar
Patzak J. 2001. Comparison of RAPD, STS, ISSR and AFLP molecular methods used for assessment of genetic diversity in hop (Humulus lupulus L.). Euphytica 121: 9 — 18.
Google Scholar
Qi L., Cao M., Chen P., Li W., Liu D. 1996. Identification, mapping, and application of polymorphic DNA associated with resistance gene Pm21 of wheat. Genome 39: 191 — 197.
Google Scholar
Qian W., Ge S., Hong D.Y. 2001. Genetic variation within and among populations of a wild rice Oryza granulata from China detected by RAPD and ISSR markers. Theor. Appl. Genet. 102: 440 — 449.
Google Scholar
Rohlf F.J. 2001. NTSYS-pc numerical taxonomy and multivariate analysis system. Version 5.1. Exeter Publishing Ltd., Setauket, N.Y.
Google Scholar
Sarla N., Neeraja C. N., Siddiq E. A. 2005. Use of anchored (AG)n and (GA)n primers to asses genetic diversity of Indian landraces and varieties of rice. Curr. Sci. 89(8): 1371 — 1381.
Google Scholar
Sztuba-Solińska J. 2005. Systemy markerów molekularnych i ich zastosowanie w hodowli roślin. Kosmos 54 (2–3): 227 — 239.
Google Scholar
Tanyolac B. 2003. Inter-simple sequence repeat (ISSR) and RAPD variation among wild barley (Hordeum vulgare subsp. spontaneum) populations from west Turkey. Genetic Resources and Crop Evolution 50: 611 — 614.
Google Scholar
Williams J. G. K., Kubelik A. R., Livak K J., Rafalski J. A., Tingey S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acid. Res. 18: 6531 — 6535.
Google Scholar
Xia G., Li Z., Wang S., Xiang F., Liu J., Chen P., Liu D. 1998. Asymmetric somatic hybridization between haploid common wheat and UV-irradiated Haynaldia villosa. Plant Sci. 137: 217 — 223.
Google Scholar
Yang Z. J., Liu C., Feng J., Li G. R., Zhou J.P., Deng K. J., Ren Z. L. 2006. Studies on genome relationship and species-specific PCR marker for Dasypyrum breviaristatum in Triticeae. Hereditas 143: 47 — 54.
Google Scholar
Yildirim A., Jones S. S., Murray T. D., Line R. F. 2000. Evaluation of Dasypyrum villosum populations for resistance to cereal eyespot and stripe rust pathogens. Plant Diseases 84: 40 — 44.
Google Scholar
Zhang Q., Li Q., Wang X., Wang H., Lang S., Wang Y., Wang S., Chen P., Liu D. 2005. Development and characterization of a Tricticum aestivum – Haynaldia villosa translocation line T4VS-4DL conferring resistance to wheat spindle streak mosaic virus. Euphytica 145: 317 — 320.
Google Scholar
Zhong G. Y., Qualset C. O. 1993. Allelic diversity of high-molecular-weight glutenin protein subunits in natural populations of Dasypyrum villosum (L.) Candargy. Theor. Appl. Genet. 86: 851 — 858.
Google Scholar
Zhong G. Y., Qualset C.O. 1995. Quantitative genetic diversity and conservation strategies for an allogamous annual species, Dasypyrum villosum. (L.) Candargy (Poaceae). Theor. Appl. Genet. 91: 1064 — 1073.
Google Scholar
Zhu Z., Tihua F., Yuan T., Ye Ch., Zhenglong R. 2007. Identification and chromosomal locations of novel genes for resistance to powdery mildew and stripe rust in wheat line 101-3. Euphytica 156: 89 — 94.
Google Scholar
Ziętkiewicz E., Rafalski A., Labuda D. 1994. Genome fingerprinting by simple-sequence repeat (SSR) – anchored chain reaction amplification. Genomics 20: 176 — 183.
Google Scholar
Authors
Agnieszka Grądzielewskagenetyka.roslin@up.lublin.pl
Instytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie Poland
Authors
Edyta Paczos-GrzędaInstytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie Poland
Authors
Daniela GruszeckaInstytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie Poland
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