Assessment of genetic similarity of spelt (Triticum aestivum ssp. spelta L.) cultivars based on RAPD markers
Sylwia Okoń
sylwia.okon@up.lublin.plInstytut Genetyki, Hodowli I Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie (Poland)
Edyta Paczos-Grzęda
Instytut Genetyki, Hodowli I Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie (Poland)
Piotr Kraska
Katedra Ekologii Rolniczej, Uniwersytet Przyrodniczy w Lublinie (Poland)
Ewa Kwiecińska-Poppe
Katedra Ekologii Rolniczej, Uniwersytet Przyrodniczy w Lublinie (Poland)
Edward Pałys
Katedra Ekologii Rolniczej, Uniwersytet Przyrodniczy w Lublinie (Poland)
Abstract
The paper contains an analysis of genetic similarity of 8 Triticum aestivum ssp. spelta cultivars done using RAPD markers. Seventeen selected primers produced 175 fragments, out of which 80 were polymorphic and 7 were genotype-specific. RAPD-based genetic similarity was estimated to be between 8.851 and 0.913. The mean genetic similarity was calculated at 0.868. Genetic similarity matrix was applied for cluster analysis through UPGMA method. The data obtained indicate that the analyzed spelt cultivars are characterized by high similarity.
Keywords:
RAPD, Triticum aestivum ssp. spelta L., genetic similarityReferences
Achleitner A., Tinker N.A., Zechner E., Buerstmayr H. 2008. Genetic diversity among oat varieties of worldwide origin and associations of AFLP markers with quantitative traits. Theor. Appl. Genet. 117: 1041 — 1053.
Google Scholar
Baumagärtel-Blaschke U. 1991. Dinkel für die neue deutsche Küche. DLG — Mitteilungen, 106, 12: 44 — 47.
Google Scholar
Cheng Z., Huang H. 2009. SSR fingerprinting Chinese peach cultivars and landraces (Prunus persica) and analysis of their genetic relationships. Scientia Hortic. 120: 188 — 193.
Google Scholar
Doyle J. J., Doyle J. L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11 — 15.
Google Scholar
Drossou A., Katsiotis A., Leggett J. M., Loukas M., Tsakas S. 2004. Genome and species relationships in genus Avena based on RAPD and AFLP molecular markers. Theor. Appl. Genet. 109: 48 — 54.
Google Scholar
Du J. K., Yao Y. Y., Ni Z. F., Peng H. R., Sun Q. X. 2002. Genetic diversity revealed by ISSR molecular marker in common wheat, spelt, compacted and progeny of recurrent selection. Acta Genet. Sin. 29 (5): 445 — 452.
Google Scholar
Grela E., Pałys E., Günther K. D. 1993. Skład chemiczny i wartość pokarmowa ziarna orkiszu (Triticum spelta) w żywieniu świń. Mat. Sympozjum Naukowego nt.: „Produkcja zwierzęca a środowisko przyrodnicze”. Wyd. AR Lublin, 214 — 222.
Google Scholar
Ikegami H., Nogata H., Hirashima K., Awamura M., Nakahara T. 2009. Analysis of genetic diversity among European and Asian fig varieties (Ficus carica L.) using ISSR, RAPD, and SSR markers. Genet. Resour. Crop Evol. 56:201 — 209.
Google Scholar
Kochieva E. Z., Goryunova S. V., Pomortsev A. A. 2001. RAPD analysis of the genome in species of the genus Hordeum. Rus. J. Genet. 37 (8): 905 — 910.
Google Scholar
Marillia E. F., Scoles G. J. 1996. The use of RAPD markers in Hordeum phylogeny. Genome 39 (4): 646 — 54.
Google Scholar
Nei M., Li W. H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci. 76: 5269 — 5273.
Google Scholar
Rohlf F. J. 2001. NTSYS-pc numerical taxonomy and multivariate analysis system. Version 5.1.Exeter Publishing Ltd., Setauket, N.Y.
Google Scholar
Sun Q., Ni Z., Liu Z., Gao J., Huang T. 1998. Genetic relationships and diversity among Tibetan wheat, common wheat and European spelt wheat revealed by RAPD markers. Euphytica 99: 205 — 211.
Google Scholar
Sztuba-Solińska J. 2005. Systemy markerów molekularnych i ich zastosowanie w hodowli roślin. Kosmos 54 (2–3): 227 — 239.
Google Scholar
Vinod K. Y., Sandeep K., Ravindra K. P. 2007. Measurement of genetic dissimilarity in fieldpea (Pisum sativum L.) genotypes using RAPD markers. Genet. Resour. Crop Evol. 54 (6): 1285 — 1289.
Google Scholar
Virk P. S., Ford-Lloyd B. V., Jackson M. T., Newbury H. J. 1995 Use of RAPD for the study of diversity within plant germplasm collections. Heredity 74: 170 — 179.
Google Scholar
Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A, Tingey S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acid. Res. 18: 6531 — 6535.
Google Scholar
Yang X., Liu P., Han Z., NI Z., Sun Q. 2005. Genetic diversity revealed by genomic-SSR and EST-SSR markers among common wheat, spelt and compacted. Progr. Nat. Sci. 15 (1):24 — 33.
Google Scholar
Authors
Sylwia Okońsylwia.okon@up.lublin.pl
Instytut Genetyki, Hodowli I Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie Poland
Authors
Edyta Paczos-GrzędaInstytut Genetyki, Hodowli I Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie Poland
Authors
Piotr KraskaKatedra Ekologii Rolniczej, Uniwersytet Przyrodniczy w Lublinie Poland
Authors
Ewa Kwiecińska-PoppeKatedra Ekologii Rolniczej, Uniwersytet Przyrodniczy w Lublinie Poland
Authors
Edward PałysKatedra Ekologii Rolniczej, Uniwersytet Przyrodniczy w Lublinie Poland
Statistics
Abstract views: 35PDF downloads: 39
License
Copyright (c) 2009 Sylwia Okoń, Edyta Paczos-Grzęda, Piotr Kraska, Ewa Kwiecińska-Poppe, Edward Pałys
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Edward Pałys, Mirosław Korzeniowski, Piotr Kraska, Beata Krusińska, Zofia Ryń, The influence of fertilization and chemical protection level on yielding of winter wheat sown after winter wheat on rendzina soil , Bulletin of Plant Breeding and Acclimatization Institute: No. 246 (2007): Regular issue
- Krzysztof Klimont, Zofia Bulińska-Radomska, Agnieszka Osińska, Grzegorz Gryziak, Józef Górka, Piotr Kraska, Reclamation efficiency of municipal sewage sludge and selected plant species for soilless post-flotation lime ground covering the surface after the borehole exploitation of sulfur , Bulletin of Plant Breeding and Acclimatization Institute: No. 278 (2015): Regular issue
- Edyta Paczos-Grzęda, Maria Chrząstek, Sylwia Okoń, Agnieszka Grądzielewska, Danuta Miazga, Application of ISSR markers in analysis of intraspecific similarity of Avena sterilis L. , Bulletin of Plant Breeding and Acclimatization Institute: No. 252 (2009): Regular issue
- Maria Chrząstek, Katarzyna Kruk, Sylwia Okoń, Edyta Paczos-Grzęda, Emilia Wójtowicz, Estimation of BC1 hybrids (Avena sativa L. × Avena maroccana Gdgr.) × Avena sativa L. regarding cytogenetic stability and some quantitative traits , Bulletin of Plant Breeding and Acclimatization Institute: No. 253 (2009): Regular issue
- Agnieszka Grądzielewska, Edyta Paczos-Grzęda, Daniela Gruszecka, Application of RAPD and ISSR methods to genetic similarity estimation of Greek Dasypyrum villosum (L.) P. Candargy populations , Bulletin of Plant Breeding and Acclimatization Institute: No. 253 (2009): Regular issue
