Allelic variation at the Xgwm261 locus in wheat cultivars (Triticum aestivum L.) registered in Poland in 1978–2006
Krzysztof Kowalczyk
krzysztof.kowalczyk@up.lublin.plInstytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie (Poland)
Sylwia Okoń
Instytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie (Poland)
Abstract
The aim of this study was an analysis of allelic variation in Xgwm 261 locus, in 45 wheat cultivars registered in Poland in the years 1978–2006. After isolation, DNA was amplified with two WMS261 primers. DNA fragments were separated on polyacrylamide gel. The analysis showed a high allelic variation in Xgwm 261 locus in these cultivars. A 174 bp DNA fragment was observed most frequently 165bp and 198bp DNA fragments were also observed. A 192 bp fragment linked with Rht8 dwarfing gene was observed in seven cultivars: Almari, Begra, Gama, Griwa, Monsun, Rada and Saga.
Keywords:
SSR, common wheat, allelic variation, Rht8 dwarfing geneReferences
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Authors
Krzysztof Kowalczykkrzysztof.kowalczyk@up.lublin.pl
Instytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie Poland
Authors
Sylwia OkońInstytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie Poland
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