Assessment of Avena fatua L. intraspecific genetic similarity based on DNA polymorphism
Edyta Paczos-Grzęda
edyta.paczos@up.lublin.plInstytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie (Poland)
Katarzyna Kruk
Instytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie (Poland)
Sylwia Okoń
Instytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie (Poland)
Abstract
The level of DNA polymorphism and genetic similarity of Polish and foreign Avena fatua L. ecotypes were studied using the ISSR and REMAP approaches. Nineteen ISSR primers and 20 REMAP primer pairs combinations used for DNA profiling, amplified 238 and 468 fragments, respectively. Polymorphic information content (PIC) values ranged from 0.52 to 0.81 for ISSR and from 0.46 to 0.99 for REMAP. Mean values of PIC for ISSR and REMAP were the same — 0.65. Genetic similarities were estimated using Dice algorithm and cluster analyses were performed using UPGMA method, independently for the two molecular marker techniques. Data obtained with both techniques were relatively high correlated (r = 0.69). Despite of high similarity of dendrograms obtained with ISSR and REMAP methods, some clustering differences were observed. Clustering did not display agreement with geographic region or country of origin. Polish ecotypes showed less diversity than forms originating from the other countries. The results of this study have provided evidence of high intra-species genetic similarity of Avena fatua.
Keywords:
Avena fatua L., REMAP, ISSR, genetic similarityReferences
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Authors
Edyta Paczos-Grzędaedyta.paczos@up.lublin.pl
Instytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie Poland
Authors
Katarzyna KrukInstytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie Poland
Authors
Sylwia OkońInstytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie Poland
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