Fusarium species and Fusarium mycotoxins in grain of barley in Poland in 2009 and 2010. Short communication
Tomasz Góral
t.goral@ihar.edu.plDepartment of Plant Pathology, Plant Breeding and Acclimatization Institute – National Research Institute, Radzików, 05-870 Błonie, Poland (Poland)
https://orcid.org/0000-0001-9130-6109
Piotr Ochodzki
Department of Plant Pathology, Plant Breeding and Acclimatization Institute – National Research Institute, Radzików, 05-870 Błonie, Poland (Poland)
https://orcid.org/0000-0003-3228-0783
Linda Kærgaard Nielsen
Sejet Plant Breeding, Nørremarksvej 67, 8700 Horsens (Denmark)
https://orcid.org/0000-0002-0976-9276
Dorota Walentyn-Góral
Department of Plant Pathology, Plant Breeding and Acclimatization Institute – National Research Institute, Radzików, 05-870 Błonie, Poland (Poland)
Abstract
Grain samples of spring barley from the 2009 and 2010 harvest were analysed for the content of DNA of Fusarium species and Fusarium toxins (type B trichothecenes). Samples originated from different fields in Radzików, Central Poland. Qualitative and quantitative determination of Fusarium species in the grain was performed using a real-time PCR. Fusarium toxins in the grain were analysed by gas chromatography. Seven Fusarium species were detected in barley grain. The dominating species were F. avenaceum, F. graminearum and F. poae. The presence of F. culmorum, F. langsethiae, F. sporotrichioides and F. tricinctum was also detected. The concentration of trichothecene toxins in grain (deoxynivalenol, nivalenol) was low. The highest correlation coefficient of deoxynivalenol vs. Fusarium DNA was found for F. graminearum. Regarding nivalenol, the highest correlation coefficient was with F. poae DNA.
Keywords:
barley, DNA, Fusarium, real-time PCR, trichothecenesReferences
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Authors
Tomasz Góralt.goral@ihar.edu.pl
Department of Plant Pathology, Plant Breeding and Acclimatization Institute – National Research Institute, Radzików, 05-870 Błonie, Poland Poland
https://orcid.org/0000-0001-9130-6109
Authors
Piotr OchodzkiDepartment of Plant Pathology, Plant Breeding and Acclimatization Institute – National Research Institute, Radzików, 05-870 Błonie, Poland Poland
https://orcid.org/0000-0003-3228-0783
Authors
Linda Kærgaard NielsenSejet Plant Breeding, Nørremarksvej 67, 8700 Horsens Denmark
https://orcid.org/0000-0002-0976-9276
Authors
Dorota Walentyn-GóralDepartment of Plant Pathology, Plant Breeding and Acclimatization Institute – National Research Institute, Radzików, 05-870 Błonie, Poland Poland
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