Polymorphism of grain storage proteins in triticale varieties registred in the Czech Republic
Tomáš Vyhnánek
int.office@mendelu.czMendel University of Agriculture and Forestry in Brno, Czech Republic (Czechia)
Jan Bednář
Mendel University of Agriculture and Forestry in Brno, Czech Republic (Czechia)
Władysław Kadłubiec
The Agriculture University of Wrocław, Poland (Poland)
Abstract
Genetic diversity was detected in 11 varieties of triticale registered in the Czech Republic by means of polymorphism of storage proteins using the PAGE ISTA method (ISTA, 1999) and SDS PAGE method (Laemmli, 1970). The polymorphism of storage proteins allowed the differentiation of the individual triticale varieties of the 2002 and 2003 harvests. On the basis of Dice’s calculations of coefficients of similarity we discovered, in parallel with the uniform genotypes, genotypes with sister prolamin spectrums with a different percentage of participation in the respective years. A uniform spectrum was detected in the following varieties: Disco, Kolor, Lamberto, Marko, Presto, Sekundo, Ticino and Tricolor; Kitaro and Modus were dimorphous varieties. In 2003 three sister prolamin lines appeared in the variety Gabo, and in 2004 only two. In 2003 a 5% admixture of a foreign genotype was detected in the variety Marko. Typical of the unknown genotype was the gliadin block Gld 1B3, which is the marker of wheat-rye translocation T1BL.1RS. The prolamin proteins of triticale grain are suitable for the detection of the genetic diversity and for the assessment of varietal authenticity and purity in seed samples of triticale varieties registered in the Czech Republic. The individual glutenin electrophoreograms were interpreted qualitatively by means of a catalogue of glutenin alleles for wheat (Payne & Lawrence, 1983). In the triticale varieties Presto, Sekundo, Tricolor, Ticino and Gabo the alleles Glu 1A2* and Glu 1B 7+8 were detected. In the varieties Kitaro, Marko and Disco the alleles Glu 1A1 and Glu 1B 7+8 were found. The heterogeneous constitution 1/2* on locus Glu 1A was detected in varieties Kitaro and Sekundo (two grains in 2003). In the varieties Kolor and Lamberto the Glu 1A0 and Glu 1B 7+8 of HMW glutenin subunits were found. Only in the variety Modus the alleles Glu 1A0 and Glu 1B 6+8 were found.
Supporting Agencies
Keywords:
triticale, X Triticosecale Wittmack, storage proteins, electrophoresisReferences
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Authors
Tomáš Vyhnánekint.office@mendelu.cz
Mendel University of Agriculture and Forestry in Brno, Czech Republic Czechia
Authors
Jan BednářMendel University of Agriculture and Forestry in Brno, Czech Republic Czechia
Authors
Władysław KadłubiecThe Agriculture University of Wrocław, Poland Poland
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Copyright (c) 2006 Tomáš Vyhnánek , Jan Bednář , Władysław Kadłubiec
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