Molecular markers linked with locus of the gene restoring male fertility in hybrids of rye with cytoplasm CMS-C
Stefan Stojałowski
dziekanat.biot@zut.edu.plKatedra Genetyki i Hodowli Roślin, Akademia Rolnicza w Szczecinie (Poland)
Paweł Milczarski
Katedra Genetyki i Hodowli Roślin, Akademia Rolnicza w Szczecinie (Poland)
Abstract
Two F2 populations of the interline crosses and a set of 62 recombinant inbred lines (RILs-F7) of rye developed from the cross 541 × Ot1-3 were analyzed during this study. The existence of linkage between formerly found three RAPD markers and the restorer locus for cytoplasm C was affirmed. Additionally, a set of five new RAPD markers linked with Rfc1 locus was identified, but only one of these markers was close enough to be attempted in using in marker assisted selection programme.
Supporting Agencies
Keywords:
RAPD, rye, cytoplasmic male sterility, genetic mappingReferences
Adolf K., Winkel A. 1985. A new source of spontaneous sterility in winter rye — preliminary results. Proc. Eucarpia Meet. Cereal Sect. on Rye, Svalöv, Sweden, 1: 293 — 306.
Google Scholar
Ahmed T. A., Tsujimoto H., Sasakuma T. 2001. QTL analysis of fertility-restoration against cytoplasmic male sterility in wheat. Genes Genet. Syst. 76: 33 — 38.
DOI: https://doi.org/10.1266/ggs.76.33
Google Scholar
Banek-Tabor A. 2004. Konstrukcja mapy genetycznej żyta przy wykorzystaniu mieszańca międzyliniowego 541 × Ot1-3 i markerów RAPD. Praca doktorska AR Szczecin.
Google Scholar
Börner A., V. Korzun, A. Polley, S. Malyshev, Melz G. 1998. Genetics and molecular mapping of male fertility restoration locus (Rfg1) in rye (Secale cereale L.). Theor. Appl. Genet. 97: 99 — 102.
DOI: https://doi.org/10.1007/s001220050871
Google Scholar
Geiger H. H., Morgenstern K. 1975. Angewandt-genetische Studien zur cytoplasmatischen Pollensterilität bei Winterroggen. Theor. Appl. Genet. 46: 269 — 276.
DOI: https://doi.org/10.1007/BF00281148
Google Scholar
Geiger H. H., Schnell F. W. 1970. Cytoplasmic male sterility in rye (Secale cereale L.). Crop Sci. 10: 590 — 593.
DOI: https://doi.org/10.2135/cropsci1970.0011183X001000050043x
Google Scholar
Kobyljanskij V. D. 1971. Sozdanie steril’nych abalogov sortov ozimoj rzi, zakrepitelej steril’nosti, vosstanovitelej fertil’nosti. Tr. Prikl. Bot. Genet. Sel. 44: 76 — 85.
Google Scholar
Lander E. S., Green P., Abrahamson J., Barlow A., Daly M. J., Lincoln S. E., Newburg L. 1987. Mapmaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174 — 181.
DOI: https://doi.org/10.1016/0888-7543(87)90010-3
Google Scholar
Łapiński M. 1972. Cytoplasmic-genic type of male sterility in Secale montanum Guss. Wheat Inform. Serv. 35: 25 — 28.
Google Scholar
Łapiński M. 1990. Dziedziczenie jałowości pyłku u międzyliniowych mieszańców żyta z cytoplazmą źródła cms-C. Hod. Rośl. Aklim. 34: 47 — 53.
Google Scholar
Melz G., Adolf K. 1991. Genetic analysis of rye (Secale cereale L.). Genetics of male sterility of the G-type. Theor. Appl. Genet. 82: 761 — 764.
DOI: https://doi.org/10.1007/BF00227322
Google Scholar
Miedaner T., C. Glass, F. Dreyer, P. Wilde, H. Wortmann, Geiger H. H. 2000. Mapping of genes for male-fertility restoration in Pampa CMS winter rye (Secale cereale L.). Theor. Appl. Genet. 101: 1226 — 1233.
DOI: https://doi.org/10.1007/s001220051601
Google Scholar
Myśków B., Masojć P., Banek-Tabor A., Szołkowski A. 2001. Genetic diversity of inbred rye lines evaluated by RAPD analysis. J. Appl. Genet. 42: 1 — 4.
Google Scholar
Stojałowski S., Łapiński M. 1997. Jądrowa determinacja cechy jałowości pyłku u międzyliniowych mieszańców żyta z cytoplazmą cms-C. "Hodowla Roślin" — materiały z I Krajowej Konferencji. Poznań, 19–20 listopada 1997: 315 — 319.
Google Scholar
Stojałowski S., Łapiński M., Masojć P. 2004. RAPD markers linked with restorer genes for C-source of cytoplasmic male sterility in rye (Secale cereale L.). Plant Breed. 123: 428 — 433.
DOI: https://doi.org/10.1111/j.1439-0523.2004.01009.x
Google Scholar
Stracke S., Schilling A. G., Förster J., Weiss C., Glass C., Miedaner T., Geiger H. H. 2003. Development of PCR-based markers linked to dominant genes for male-fertility restoration in Pampa CMS of rye (Secale cereale L.). Theor. Appl. Genet. 106: 1184 — 1190.
DOI: https://doi.org/10.1007/s00122-002-1153-4
Google Scholar
Thomson D., Henry R., 1995. Single-step protocol for preparation of plant tissue for analysis by PCR. BioTechniques 19: 394 — 400.
Google Scholar
Authors
Stefan Stojałowskidziekanat.biot@zut.edu.pl
Katedra Genetyki i Hodowli Roślin, Akademia Rolnicza w Szczecinie Poland
Authors
Paweł MilczarskiKatedra Genetyki i Hodowli Roślin, Akademia Rolnicza w Szczecinie Poland
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