The molecular analysis of male-sterile, maintainer and restorer lines of rye
Andrzej Rafalski
a.rafalski@ihar.edu.plZakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Magdalena Żurawska
Zakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Irena Kolasińska
Zakład Genetyki i Hodowli Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Iwona Wiśniewska
Zakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Abstract
The molecular analysis included male-sterile (P), their analogous maintainer (N) and restorer (R) inbred lines of rye originating from the Plant Breeding and Acclimatisation Institute, Danko Plant Breeding Co. Ltd. and Poznań Plant Breeding Co. Ltd. Presented data are the results of a preliminary study on identification of markers of fertility restoration for male sterility of "Pampa" type. The aim of this study was to document the utility of proposed PCR technique for differentiation the components of rye hybrids: male sterile, maintainer and restorer lines. The use of considerable number of starters gave also a possibility to select semi-specific primers that seem to be the most useful for further studies. The evaluation of indices of dissimilarity among accessions and cluster data was based on the analysis of 1482 DNA fragments. The results indicated essential genetic differences between restorer and non-restorer inbred lines. The data presented also confirmed a high level of similarity between male sterile lines and their normal analogues.
Keywords:
heterosis, inbred lines, markers of restoration, semi-specific PCR, ryeReferences
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Authors
Andrzej Rafalskia.rafalski@ihar.edu.pl
Zakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Magdalena ŻurawskaZakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Irena KolasińskaZakład Genetyki i Hodowli Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Iwona WiśniewskaZakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
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Copyright (c) 2005 Andrzej Rafalski, Magdalena Żurawska, Irena Kolasińska, Iwona Wiśniewska
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