Usefulness of ISSR markers for identification of inbred lines and mapping of rye genome
Stefan Stojałowski
dziekanat.biot@zut.edu.plKatedra Genetyki i Hodowli Roślin, Akademia Rolnicza w Szczecinie (Poland)
Paweł Milczarski
Katedra Genetyki i Hodowli Roślin, Akademia Rolnicza w Szczecinie (Poland)
Piotr Masojć
Katedra Genetyki i Hodowli Roślin, Akademia Rolnicza w Szczecinie (Poland)
Abstract
This study was aimed at evaluation of the utility of ISSR markers as a tool for identification of inbred lines and for construction of genetic map of rye. Sixteen rye inbred lines and a mapping population composed of 94 individual F2 plants from the 544 × Ot0-20 intercross were used for the study. Twelve primers, which generated strong and reproducible amplification products, were selected out of 57 primers tested. The selected primers revealed 29 polymorphic bands which allowed the identification of lines and the estimation of their genetic diversity. A dendrogram developed on the basis of genetic similarities proved to be generally consistent with the pedigree data. Out of eight markers used for the analysis of F2 intercross population, six showed segregation consistent with 3:1 expectation for a single locus. The presented results of ISSR marker segregation suggest that this PCR-based method should be suitable for construction of rye genetic map. However, its efficiency seems to be comparable with that of RAPD.
Supporting Agencies
Keywords:
ISSR, genetic mapping, molecular markers, ryeReferences
Ammiraju J. S. S., Dholakia B. B., Dantra D. K., Sigh H., Lagu M. D., Tamhankar S. A., Dhaliwal H. S., Rao V. S., Gupta V. S., Ranjekar P. K. 2001. Identification of inter simple sequence repeat (ISSR) markers associated with seed size in wheat. Theor. Appl. Genet. 102: 726 — 732.
DOI: https://doi.org/10.1007/s001220051703
Google Scholar
Blair M. W., Panaud O., McCouch S. R. 1999. Inter-simple sequence repeat (ISSR) amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza sativa L.). Theor. Appl. Genet. 98: 780 — 792.
DOI: https://doi.org/10.1007/s001220051135
Google Scholar
Cekic C., Battey N. H., Wilkinson M. J. 2001. The potential of ISSR-PCR primer-pair combinations for genetic linkage analysis using the seasonal flowering locus in Fragaria as a model. Theor. Appl. Genet. 103: 540 — 546.
DOI: https://doi.org/10.1007/PL00002907
Google Scholar
Fernandez M. E., Figueiras A. M., Benito C. 2002. The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among cultivars with known origin. Theor. Appl. Genet. 104: 845 — 851.
DOI: https://doi.org/10.1007/s00122-001-0848-2
Google Scholar
Kantety R. V., Zeng X. P., Bennetzen J. L., Zehr B. E. 1995. Assessment of genetic diversity in dent and popcorn (Zea mays L.) inbred lines using inter-simple sequence repeat (ISSR) amplification. Mol. Breed. 1: 365 — 373.
DOI: https://doi.org/10.1007/BF01248414
Google Scholar
Kojima T., Nagaoka T., Noda K., Ogihara Y. 1998. Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers. Theor. Appl. Genet. 96: 37 — 45.
DOI: https://doi.org/10.1007/s001220050706
Google Scholar
Masojć P., Myśków B., Milczarski P. 2001. Extending a RFLP-based genetic map of rye using random amplified polymorphic DNA (RAPD) and isozyme markers. Theor. Appl. Genet. 102: 1273 — 1279.
DOI: https://doi.org/10.1007/s001220000512
Google Scholar
Moreno S., Martin J. P., Ortiz J. M. 1998. Inter-simple sequence repeats PCR for characterization of closely related grapevine germplasm. Euphytica 101: 117 — 125.
DOI: https://doi.org/10.1023/A:1018379805873
Google Scholar
Myśków B., Masojć P., Banek-Tabor A., Szołkowski A. 2001. Genetic diversity of inbred rye lines evaluated by RAPD analysis. J. Appl. Genet. 42: 1 — 4.
Google Scholar
Nagoaka T., Ogihara Y. 1997. Applicability of inter-simple sequence repeat polymorphism in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theor. Appl. Genet. 94: 597 — 602.
DOI: https://doi.org/10.1007/s001220050456
Google Scholar
Pradeep Reddy M., Sarla N., Siddiq E. A. 2002. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128: 9 — 17.
DOI: https://doi.org/10.1023/A:1020691618797
Google Scholar
Ratnaparkhe M. B., Tekeoglu M., Muehlbauer F. J. 1998. Inter-simple sequence repeat (ISSR) polymorphisms are useful for finding markers associated with disease resistance gene clusters. Theor. Appl. Genet. 97: 515 — 519.
DOI: https://doi.org/10.1007/s001220050925
Google Scholar
Smolik M., Rzepka-Plevneš D. 2002. Wykorzystanie polimorfizmu DNA w regionach sekwencji mikrosatelitarnych do identyfikacji genotypów żyta o różnej tolerancji na niedobory pokarmowe w podłożu. Zesz. Probl. Post. Nauk Rol. 488 „Nowoczesne metody i techniki w hodowli roślin”: 145 — 151.
Google Scholar
Thomson D., Henry R. 1995. Single-step protocol for preparation of plant tissue for analysis by PCR. BioTechniques 19: 394 — 400.
Google Scholar
Virk P. S., Zhu J., Newbury H. J., Bryan G. J., Jackson M. T., Ford-Lloyd B. V. 2000. Effectiveness of different classes of molecular marker for classifying and revealing variation in rice (Oryza sativa) germplasm. Euphytica 112: 275 — 284.
DOI: https://doi.org/10.1023/A:1003952720758
Google Scholar
Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531 — 6535.
DOI: https://doi.org/10.1093/nar/18.22.6531
Google Scholar
Ziętkiewicz E., Rafalski A., Labuda D. 1994. Genome fingerprinting by simple sequence repeat (SSR) — Anchored Polymerase Chain Reaction Amplification. Genomics 20: 176 — 183.
DOI: https://doi.org/10.1006/geno.1994.1151
Google Scholar
Authors
Stefan Stojałowskidziekanat.biot@zut.edu.pl
Katedra Genetyki i Hodowli Roślin, Akademia Rolnicza w Szczecinie Poland
Authors
Paweł MilczarskiKatedra Genetyki i Hodowli Roślin, Akademia Rolnicza w Szczecinie Poland
Authors
Piotr MasojćKatedra Genetyki i Hodowli Roślin, Akademia Rolnicza w Szczecinie Poland
Statistics
Abstract views: 81PDF downloads: 28
License
Copyright (c) 2004 Stefan Stojałowski, Paweł Milczarski, Piotr Masojć
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Mirosław Tyrka , Grzegorz Fic , Magdalena Szeliga , Marcin Jaromin , Paweł Krajewski , Paweł Milczarski , Tadeusz Drzazga , Przemysław Matysik , Róża Mazur , Teresa Sikora , Edward Witkowski , Justyna Buczkowicz , Dorota Tyrka , Genomic selection of winter wheat , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Stefan Stojałowski , Marta Orłowska , Martyna Sobczyk , Anna Bienias , Marcin Berdzik , Beata Myśków , Halina Góral , Magdalena Simlat , Tomasz Warzecha , Wojciech Wesołowski , Marek Szklarczyk , Mirosław Pojmaj , Genetic background of male sterility in triticale with different cytoplasm’s and perspectives of it utilization for development of CMS system in wheat , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Stefan Stojałowski , Marta Orłowska , Martyna Sobczyk , Anna Bienias , Beata Myśków , Przemysław Tomczak , Wojciech Wesołowski , Marek Szklarczyk , Waldemar Brukwiński , Katarzyna Banaszak, Monika Hanek, Renata Krysztrofik, Marek Zając, Search of common genetic mechanisms determining fertility of plants with cytoplasm CMS-C and CMS-Pampa , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Stefan Stojałowski , Marta Orłowska , Martyna Sobczyk , Anna Bienias , Beata Myśków , Marzena Ostrowska , Marek Zając , Róża Mazur , Dorota Jasińska , Study on internal genetic structure of rye cultivars and genetic background of heterosis , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Monika Kociuba, Stefan Stojałowski, Efficiency of the SCAR markers from 4RL chromosome in selection of male fertile genotypes and their relation to some morphological traits in rye (Secale cereale L.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 252 (2009): Regular issue
- Anetta Kuczyńska, Jan Bocianowski, Piotr Masojć, Maria Surma, Tadeusz Adamski, Genetic similarity among cultivars of winter barley (Hordeum vulgare L.) revealed by random amplified polymorphic DNA (RAPD) , Bulletin of Plant Breeding and Acclimatization Institute: No. 226/227 (2003): Regular issue
- Marek Szklarczyk, Wojciech Wesołowski, Marcelina Wajdzik, Anna Szlachtowska, Beata Domnicz, Stefan Stojałowski, Identification of genes responsible for fertility restoration and self-compatibility in selected vegetable plants , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Paweł Milczarski, Identification of QTLs of morphological traits related to lodging of rye (Secale cereale L.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 250 (2008): Regular issue
- Stefan Stojałowski, Polymorphism of STS and SSR markers in rye inbred lines , Bulletin of Plant Breeding and Acclimatization Institute: No. 244 (2007): Regular issue
- Magdalena Pol-Szyszko, Piotr Masojč, Izabela Doniec, Agnieszka Wenda, Analysis of α-amylase activity in grain of maize (Zea mays L.) cultivars in respect of their application to bioethanol production , Bulletin of Plant Breeding and Acclimatization Institute: No. 254 (2009): Regular issue
