Molecular and cytogenetic analysis and estimation of some quantitative traits of interspecific hybrids Avena sativa L. × Avena fatua L.
Maria Chrząstek
dziekanat.agbioinz@up.lublin.plInstytut Genetyki i Hodowli Roślin, Akademia Rolnicza, Lublin (Poland)
Edyta Paczos-Grzęda
Instytut Genetyki i Hodowli Roślin, Akademia Rolnicza, Lublin (Poland)
Abstract
The aim of the presented paper were genetic and breeding studies of F2 interspecific hybrids Avena sativa L. cv. Borys × Avena fatua L. and the parental forms. Hybrid character of this cross combination was confirmed using the RAPD (Random Amplified Polymorphic DNA) method. Configurations of chromosomes in pollen mother cells (PMCs) at methaphase I and anaphase I, frequency of micronuclei in tetrads, viability and size of pollen grains were analysed in order to estimate the cytogenetic stability of hybrids. At metaphase I mainly bivalents were observed and at anaphase I rarely lagging chromosomes and chromatid bridges were noted. In the hybrids, as well as in the initial forms, tetrads were properly formed and pollen was highly viable. The obtained results testify to good stability of the analysed genotypes. The hybrids were compared to their parental forms regarding some quantitative traits. In majority of cases significant differences were stated. In comparison with the Borys variety, hybrids were higher and had longer panicles. Number of spikelets per panicle, as well as number and weight of kernels, were lower than in the parental forms. Higher fertility and total protein content in kernels, but at the same time lower weight of 1000 kernels were stated.
Keywords:
Avena sativa L., Avena fatua L., RAPD, interspecific hybrids, meiosis, PMC, quantitative traitsReferences
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Authors
Maria Chrząstekdziekanat.agbioinz@up.lublin.pl
Instytut Genetyki i Hodowli Roślin, Akademia Rolnicza, Lublin Poland
Authors
Edyta Paczos-GrzędaInstytut Genetyki i Hodowli Roślin, Akademia Rolnicza, Lublin Poland
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