The influence of inoculation with fungi of the genus Fusarium on beta-sitosterol content in grain of different varieties of winter wheat
Tomasz Góral
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików (Poland)
Kinga Stuper-Szablewska
kstuper@up.poznan.plUniwersytet Przyrodniczy, Katedra Chemii (Poland)
Anna Przybylska
Uniwersytet Przyrodniczy, Katedra Chemii (Poland)
Anna Matysik
Uniwersytet Przyrodniczy, Katedra Chemii (Poland)
Juliusz Perkowski
Uniwersytet Przyrodniczy, Katedra Chemii (Poland)
Abstract
The aim of the study was to determine the effect of inoculation with Fusarium fungi on the content of beta-sitosterol in wheat grain. Thirty varieties of winter wheat cultivated under the same agronomic and climatic conditions were tested in two experimental variants. The first of these was a control trial growing without interference, while the second variant was an inoculation experiment with fungal spores of the genus Fusarium. Beta-sitosterol, the most important among the endogenous sterols of plants, was analyzed after microwave assisted saponification and pentane extraction with UPLC with DAD detector. Based on the results of the chemical analyzes, significant differences in sterol contents between control and inoculated samples as well as between varieties were found. The mean concentration of beta-sitosterol in the control samples was 433.51 mg/kg, whereas in the inoculated samples it was significantly lower and was 340.88 mg/kg. This study clearly indicates that the mechanism of metabolism of plant sterols presented in the literature for the other microorganisms is similar to that of Fusarium species.
Supporting Agencies
Keywords:
beta-sitosterol, Fusarium, wheatReferences
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Authors
Tomasz GóralInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików Poland
Authors
Kinga Stuper-Szablewskakstuper@up.poznan.pl
Uniwersytet Przyrodniczy, Katedra Chemii Poland
Authors
Anna PrzybylskaUniwersytet Przyrodniczy, Katedra Chemii Poland
Authors
Anna MatysikUniwersytet Przyrodniczy, Katedra Chemii Poland
Authors
Juliusz PerkowskiUniwersytet Przyrodniczy, Katedra Chemii Poland
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