Identification of the genetic basis of germination in rape seed (Brassica napus L.) using genetic mapping
Katarzyna Gacek
kgacek@nico.ihar.poznan.plInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu (Poland)
https://orcid.org/0000-0001-8621-8673
Iwona Bartkowiak-Broda
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu (Poland)
https://orcid.org/0000-0002-4071-1849
Laurencja Szala
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu (Poland)
https://orcid.org/0000-0001-9157-7638
Teresa Cegielska-Taras
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu (Poland)
https://orcid.org/0000-0002-6002-9281
Philipp E. Bayer
School of Plant Biology, University of Western Australia, Perth, Crawley, Australia (Australia)
https://orcid.org/0000-0001-8530-3067
David Edwards
School of Plant Biology, University of Western Australia, Perth, Crawley, Australia (Australia)
https://orcid.org/0000-0002-9480-4936
Jacqueline Batley
School of Plant Biology, University of Western Australia, Perth, Crawley, Australia (Australia)
https://orcid.org/0000-0002-5391-5824
Steven Penfield
John Innes Centre, Norwich, UK (United Kingdom)
https://orcid.org/0000-0001-6340-1731
Abstract
The mapping population of winter oilseed rape composed of 78 doubled haploid lines obtained from hybrids originating from the intersection of lines differing in germination power was examined. Seed germination power was automatically recorded every 30 minutes using a camera placed in the Raspberry Pi mini computer. The photos were analyzed using software created at the John Innes Center. In the next step, using the whole genome sequencing method (Illumina® HiSeq) of all mapping population lines, single nucleotide polymorphisms (SNPs) were identified in these lines. This data will be used for genetic mapping and identification of genes regulating the germination process.
Supporting Agencies
The authors would like to acknowledge funding support from the National Science Centre, Poland (Project 2017/01/X/NZ2/00113).
Keywords:
rape seed, genetic mapping, germinationGacek, K. . (2019) “Identification of the genetic basis of germination in rape seed (Brassica napus L.) using genetic mapping ”, Bulletin of Plant Breeding and Acclimatization Institute, (287), pp. 23–24. doi: 10.37317/biul-2019-0100.
Authors
Katarzyna Gacekkgacek@nico.ihar.poznan.pl
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu Poland
https://orcid.org/0000-0001-8621-8673
Authors
Iwona Bartkowiak-BrodaInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu Poland
https://orcid.org/0000-0002-4071-1849
Authors
Laurencja SzalaInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu Poland
https://orcid.org/0000-0001-9157-7638
Authors
Teresa Cegielska-TarasInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Poznaniu Poland
https://orcid.org/0000-0002-6002-9281
Authors
Philipp E. BayerSchool of Plant Biology, University of Western Australia, Perth, Crawley, Australia Australia
https://orcid.org/0000-0001-8530-3067
Authors
David EdwardsSchool of Plant Biology, University of Western Australia, Perth, Crawley, Australia Australia
https://orcid.org/0000-0002-9480-4936
Authors
Jacqueline BatleySchool of Plant Biology, University of Western Australia, Perth, Crawley, Australia Australia
https://orcid.org/0000-0002-5391-5824
Authors
Steven PenfieldJohn Innes Centre, Norwich, UK United Kingdom
https://orcid.org/0000-0001-6340-1731
Statistics
Abstract views: 234PDF downloads: 162
License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Laurencja Szała, Zygmunt Kaczmarek, Elżbieta Adamska, Teresa Cegielska-Taras, Influence of the direction of crossing on the expression of seed colour and yield components in DH line populations of winter oilseed rape , Bulletin of Plant Breeding and Acclimatization Institute: No. 264 (2012): Regular issue
- Teresa Cegielska-Taras, Katarzyna Sosnowska, Laurencja Szała , Introduction of new alleles from gene pools of various Brassica species to the winter oilseed rape genome , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Błażej Hernacki, Iwona Bartkowiak-Broda, Aleksandra Piotrowska, Teresa Cegielska-Taras, A consideration on genetic mapping of QTL responsible for the yellow-seedness in winter rapeseed (Brassica napus L.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 253 (2009): Regular issue
- Jan Bocianowski, Alina Lieesch, Iwona Bartkowiak-Broda, Wiesława Popławska, Characteristics of winter oilseed rape (Brassica napus L.) volunteers with the use of RAPD markers , Bulletin of Plant Breeding and Acclimatization Institute: No. 249 (2008): Regular issue
- Zygmunt Kaczmarek, Laurencja Szała, Elżbieta Adamska, Teresa Cegielska-Taras, Statistical and genetic evaluation of winter oilseed rape DH lines based on the results of an unreplicated experiment with standards , Bulletin of Plant Breeding and Acclimatization Institute: No. 253 (2009): Regular issue
- Iwona Bartkowiak-Broda , Alina Liersch, Marcin Matuszczak, Katarzyna Mikołajczyk, Wiesława Popławska, Joanna Wolko, Joanna Nowakowska, The investigations of winter rapeseed genome with the use of molecular markers , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Alina Lieesch, Wiesława Popławska, Maria Ogrodowczyk, Iwona Bartkowiak-Broda, Jan Bocianowski, Phenotypic characteristics of winter oilseed rape volunteers (Brassica napus L.) occurring in northern regions of Poland , Bulletin of Plant Breeding and Acclimatization Institute: No. 250 (2008): Regular issue
- Alina Lieesch, Jan Bocianowski, Iwona Bartkowiak-Broda, Search for molecular markers linked to the flowering time and length of parental lines of F1 CMS ogura hybrids of winter oilseed rape (B. napus L.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 264 (2012): Regular issue
- Iwona Bartkowiak-Broda, Memory - Prof. dr hab. Jan Krzymański , Bulletin of Plant Breeding and Acclimatization Institute: No. 299 (2023): Regular issue
- Zygmunt Kaczmarek, Elżbieta Adamska, Laurencja Szała, Teresa Cegielska-Taras, Stability of combining ability for unsaturated fatty acids content in DH lines of winter oilseed rape , Bulletin of Plant Breeding and Acclimatization Institute: No. 260/261 (2011): Regular issue
