Use of molecular and phenotypic markers to identify wheat eyespot resistance genes caused by Oculimacula yallundae and O. acuformis

Maciej Majka


Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0002-5039-9465

Magdalena Gawłowska


Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0002-8975-0208

Adriana Twardawska


Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0003-1139-8133

Marek Korbas


Instytut Ochrony Roślin — Państwowy Instytut Badawczy, Władysława Węgorka 20, 60-318 Poznań (Poland)
https://orcid.org/0000-0002-5536-1853

Jakub Danielewicz


Instytut Ochrony Roślin — Państwowy Instytut Badawczy, Władysława Węgorka 20, 60-318 Poznań (Poland)
https://orcid.org/0000-0003-0252-8573

Tomasz Góral


Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, 05-870 Błonie (Poland)
https://orcid.org/0000-0001-9130-6109

Bogusława Ługowska


DANKO Hodowla Roślin, Choryń 27, 64-000 Kościan (Poland)

Jolanta Belter


Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0003-1934-448X

Edward Witkowski


Hodowla Roślin Smolice, Grupa IHAR, Smolice 134, 63-740 Kobylin (Poland)

Tadeusz Drzazga


Małopolska Hodowla Roślin, ul. Sportowa 21, 55-040 Kobierzyce (Poland)

Przemysław Matysik


Hodowla Roślin Strzelce, Grupa IHAR, Główna 20, 99-307 Strzelce (Poland)

Urszula Woźna-Pawlak


Poznańska Hodowla Roślin, Antoniny 2, 64-100 Leszno (Poland)
https://orcid.org/0000-0002-1454-6181

Halina Wiśniewska

hwis@igr.poznan.pl
Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0003-2782-685X

Abstract

Eyespot is one of the most important diseases of wheat (Triticum aestivum L.), caused by two pathogenic fungi Oculimacula yallundae and Oculimacula acuformis. There are several sources of resistance to this pathogen, but so far only two genes Pch1 and Pch2 have been transferred to wheat and confer the resistance. The selection of the most favorable genetic markers for determining the presence of eyespot resistance genes may improve the efficiency and accuracy in the selection of wheat genotypes resistant for this disease. The aim of the work was to determine the effectiveness of molecular markers and protein marker for Pch1 and Pch2 genes and to select genotypes of winter wheat with increased resistance in relation to plant infection in inoculation tests carried out at the seedling and adult plant stage. Plant material consist of 159 breeding lines of winter wheat and five control varieties: Artist, Kilimanjaro, Kometa, Patras and Rendezvous. To identify resistant genes, five molecular markers were used. Three for Pch1 gene (EpD1b, XustSSR2001-7DL, Xorw1) and two for Pch2 gene (Xcfa2040, Xwmc525). Considering the molecular analysis of genes, results of seedlings inoculation and the results of plant stem infestation, it was found that there were no infection symptoms of stalks in the winter wheat lines and/or varieties, in which both Pch1 and Pch2 genes were identified. In these lines/varieties, the lowest infection of seedlings was also observed. The highest percentage of infected sheaths was identified in genotypes where Pch1 and Pch2 genes were not found. The highest seedling infection was also observed for these genotypes. It was shown that the presence of Pch1 and Pch2 genes or their absence did not significantly affect the grain yield and the thousands kernels weight (TKW). Genotypes with the Pch1 and Pch2 genes showed slightly higher values for both technological parameters.

 

Supporting Agencies

Badania finansowano ze środków projektu MRiRW: Badania Podstawowe na rzecz Postępu Biologicznego w Produkcji Roślinnej; decyzja HOR.hn.802.18.2018 lp.2. Majka M. jest stypendystą korzystającym ze wsparcia finansowego Fundacji na rzecz Nauki Polskiej (FNP).

Keywords:

resistance genes, eyespot, Pch1, Pch2, Triticum aestivum

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Published
2020-02-12

Cited by

Majka, M. (2020) “Use of molecular and phenotypic markers to identify wheat eyespot resistance genes caused by Oculimacula yallundae and O. acuformis”, Bulletin of Plant Breeding and Acclimatization Institute, (288), pp. 3–14. doi: 10.37317/biul-2020-0001.

Authors

Maciej Majka 

Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0002-5039-9465

Authors

Magdalena Gawłowska 

Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0002-8975-0208

Authors

Adriana Twardawska 

Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0003-1139-8133

Authors

Marek Korbas 

Instytut Ochrony Roślin — Państwowy Instytut Badawczy, Władysława Węgorka 20, 60-318 Poznań Poland
https://orcid.org/0000-0002-5536-1853

Authors

Jakub Danielewicz 

Instytut Ochrony Roślin — Państwowy Instytut Badawczy, Władysława Węgorka 20, 60-318 Poznań Poland
https://orcid.org/0000-0003-0252-8573

Authors

Tomasz Góral 

Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, 05-870 Błonie Poland
https://orcid.org/0000-0001-9130-6109

Authors

Bogusława Ługowska 

DANKO Hodowla Roślin, Choryń 27, 64-000 Kościan Poland

Authors

Jolanta Belter 

Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0003-1934-448X

Authors

Edward Witkowski 

Hodowla Roślin Smolice, Grupa IHAR, Smolice 134, 63-740 Kobylin Poland

Authors

Tadeusz Drzazga 

Małopolska Hodowla Roślin, ul. Sportowa 21, 55-040 Kobierzyce Poland

Authors

Przemysław Matysik 

Hodowla Roślin Strzelce, Grupa IHAR, Główna 20, 99-307 Strzelce Poland

Authors

Urszula Woźna-Pawlak 

Poznańska Hodowla Roślin, Antoniny 2, 64-100 Leszno Poland
https://orcid.org/0000-0002-1454-6181

Authors

Halina Wiśniewska 
hwis@igr.poznan.pl
Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0003-2782-685X

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