Use of molecular and phenotypic markers to identify wheat eyespot resistance genes caused by Oculimacula yallundae and O. acuformis
Maciej Majka
Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0002-5039-9465
Magdalena Gawłowska
Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0002-8975-0208
Adriana Twardawska
Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0003-1139-8133
Marek Korbas
Instytut Ochrony Roślin — Państwowy Instytut Badawczy, Władysława Węgorka 20, 60-318 Poznań (Poland)
https://orcid.org/0000-0002-5536-1853
Jakub Danielewicz
Instytut Ochrony Roślin — Państwowy Instytut Badawczy, Władysława Węgorka 20, 60-318 Poznań (Poland)
https://orcid.org/0000-0003-0252-8573
Tomasz Góral
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, 05-870 Błonie (Poland)
https://orcid.org/0000-0001-9130-6109
Bogusława Ługowska
DANKO Hodowla Roślin, Choryń 27, 64-000 Kościan (Poland)
Jolanta Belter
Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0003-1934-448X
Edward Witkowski
Hodowla Roślin Smolice, Grupa IHAR, Smolice 134, 63-740 Kobylin (Poland)
Tadeusz Drzazga
Małopolska Hodowla Roślin, ul. Sportowa 21, 55-040 Kobierzyce (Poland)
Przemysław Matysik
Hodowla Roślin Strzelce, Grupa IHAR, Główna 20, 99-307 Strzelce (Poland)
Urszula Woźna-Pawlak
Poznańska Hodowla Roślin, Antoniny 2, 64-100 Leszno (Poland)
https://orcid.org/0000-0002-1454-6181
Halina Wiśniewska
hwis@igr.poznan.plInstytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0003-2782-685X
Abstract
Eyespot is one of the most important diseases of wheat (Triticum aestivum L.), caused by two pathogenic fungi Oculimacula yallundae and Oculimacula acuformis. There are several sources of resistance to this pathogen, but so far only two genes Pch1 and Pch2 have been transferred to wheat and confer the resistance. The selection of the most favorable genetic markers for determining the presence of eyespot resistance genes may improve the efficiency and accuracy in the selection of wheat genotypes resistant for this disease. The aim of the work was to determine the effectiveness of molecular markers and protein marker for Pch1 and Pch2 genes and to select genotypes of winter wheat with increased resistance in relation to plant infection in inoculation tests carried out at the seedling and adult plant stage. Plant material consist of 159 breeding lines of winter wheat and five control varieties: Artist, Kilimanjaro, Kometa, Patras and Rendezvous. To identify resistant genes, five molecular markers were used. Three for Pch1 gene (EpD1b, XustSSR2001-7DL, Xorw1) and two for Pch2 gene (Xcfa2040, Xwmc525). Considering the molecular analysis of genes, results of seedlings inoculation and the results of plant stem infestation, it was found that there were no infection symptoms of stalks in the winter wheat lines and/or varieties, in which both Pch1 and Pch2 genes were identified. In these lines/varieties, the lowest infection of seedlings was also observed. The highest percentage of infected sheaths was identified in genotypes where Pch1 and Pch2 genes were not found. The highest seedling infection was also observed for these genotypes. It was shown that the presence of Pch1 and Pch2 genes or their absence did not significantly affect the grain yield and the thousands kernels weight (TKW). Genotypes with the Pch1 and Pch2 genes showed slightly higher values for both technological parameters.
Supporting Agencies
Keywords:
resistance genes, eyespot, Pch1, Pch2, Triticum aestivumReferences
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Authors
Maciej MajkaInstytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0002-5039-9465
Authors
Magdalena GawłowskaInstytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0002-8975-0208
Authors
Adriana TwardawskaInstytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0003-1139-8133
Authors
Marek KorbasInstytut Ochrony Roślin — Państwowy Instytut Badawczy, Władysława Węgorka 20, 60-318 Poznań Poland
https://orcid.org/0000-0002-5536-1853
Authors
Jakub DanielewiczInstytut Ochrony Roślin — Państwowy Instytut Badawczy, Władysława Węgorka 20, 60-318 Poznań Poland
https://orcid.org/0000-0003-0252-8573
Authors
Tomasz GóralInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, 05-870 Błonie Poland
https://orcid.org/0000-0001-9130-6109
Authors
Bogusława ŁugowskaDANKO Hodowla Roślin, Choryń 27, 64-000 Kościan Poland
Authors
Jolanta BelterInstytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0003-1934-448X
Authors
Edward WitkowskiHodowla Roślin Smolice, Grupa IHAR, Smolice 134, 63-740 Kobylin Poland
Authors
Tadeusz DrzazgaMałopolska Hodowla Roślin, ul. Sportowa 21, 55-040 Kobierzyce Poland
Authors
Przemysław MatysikHodowla Roślin Strzelce, Grupa IHAR, Główna 20, 99-307 Strzelce Poland
Authors
Urszula Woźna-PawlakPoznańska Hodowla Roślin, Antoniny 2, 64-100 Leszno Poland
https://orcid.org/0000-0002-1454-6181
Authors
Halina Wiśniewskahwis@igr.poznan.pl
Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0003-2782-685X
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