The application of RAPD markers to estimation of genotypic diversity of Polish winter triticale cultivars
Aneta Kramek
aneta.kramek@up.lublin.plInstytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie (Poland)
Abstract
The aim of this study was estimation of genotypic diversity of 22 Polish winter triticale cultivars based on RAPD markers. The estimation of intercultivar polymorphism was carried out basing on 15 RAPD primers, which were selected among 48 primers tested on 5 winter triticale genotypes. The chosen oligonucleotides generated 135 fragments of DNA, 87 of them (64.44%) were polymorphic. The mean value of similarity index was 0.862 and fluctuated from 0.734 between cv. Malno and cv. Tewo to 0.903 between cv. Marko and cv. Fidelio. The highest average genetic distance to all examined cultivars was observed in the cv. Tewo (0.785), the cv. Fidelio was the most similar to other cultivars. On the base of obtained results a low level of genotypic diversity was stated among the studied winter triticale cultivars.
Keywords:
genotypic diversity, RAPD markers, triticaleReferences
Broda Z. 2000. Diagnostyka molekularna w badaniach zmienności genetycznej roślin. Hod. Rośl. Nasien. 4: 36 — 37.
Google Scholar
Broda Z., Wojciechowska A. 2004. Przyszłość hodowli roślin w diagnostyce molekularnej. Hod. Rośl. Nasien. 2: 2 — 4.
Google Scholar
González J.M., Jouve N., Gustafson J. P., Muńiz L. M. 2002. A genetic map of molecular markers in × Triticosecale Wittmack. In: Proc. of the 5th Int. Triticale Symp., IHAR Radzików, Poland, 30 June — 5 July 2002, vol. II: 85 — 93.
Google Scholar
Iqbal J. M., Aziz N., Saeed N. A., Zafar Y. 1997. Genetic diversity evaluation of some elite cotton varieties by RAPD analysis. Theor. Appl. Genet. 94: 139 — 144.
Google Scholar
Lista Odmian Roślin Uprawnych, COBORU, Słupia Wielka: 1982–2003.
Google Scholar
Masojć P. 2000. Identyfikacja odmian pszenżyta przy użyciu markerów RAPD. Folia Univ. Agric. Stetin., Agricultura 206 (82): 179 — 184.
Google Scholar
Milczarski P., Banek-Tabor A., Masojć P. 2001. Wykorzystanie markerów RAPD do identyfikacji odmian pszenżyta. Biul. IHAR 218/219: 261 — 267.
Google Scholar
Milligan B.G. 1992. Molecular analysis of populations: a practical approach. Plant DNA isolation. IRL Press, Oxford, UK: 59 — 88.
Google Scholar
Nei M., Li W. H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269 — 5273.
Google Scholar
Rohlf F. J. 2001. NTSYS-pc numerical taxonomy and multivariate analysis system. Version 2.10q. Exeter Publishing Ltd., Setauket, N.Y: 171.
Google Scholar
Schut J.W., Qi X., Stam P. 1997. Association between relationship measures based on AFLP markers, pedigree data and morphological traits in barley. Theor. Appl. Genet. 95: 1161 — 1168.
Google Scholar
Stojałowski S., Góral H. 2002. Wykorzystanie markerów RAPD i ISSR do różnicowania linii CMS pszenżyta ozimego z cytoplazmą T. timopheevi. Folia Univ. Agric. Stetin., Agricultura 228 (91): 161 — 166.
Google Scholar
Tams S. H., Bauer E., Oettler G., Melchinger A. E. 2004. Genetic diversity in European winter triticale determined with SSR markers and coancestry coefficient. Theor. Appl. Genet. 108: 1385 — 1391.
Google Scholar
Tams S. H., Melchinger A. E., Bauer E. 2005. Genetic similarity among European winter triticale elite germplasm assessed with AFLP and comparisons with SSR and pedigree data. Plant Breed. 124: 154 — 160.
Google Scholar
Tams S. H., Melchinger A. E., Oettler G., Bauer E. 2002. Assessment of genetic diversity in European winter triticale using molecular markers and pedigree data. In: Proc. of the 5th Int. Triticale Symp., IHAR Radzików, Poland, 30 June –— 5 July. vol. I: 95 — 103.
Google Scholar
Terzi V. 1998. RAPD markers for fingerprinting barley, oat and triticale varieties. J. Genet. Breed. 51(2): 115 — 120.
Google Scholar
Twardowska M., Banek-Tabor A., Masojć P. 2002. Identyfikacja odmian pszenżyta ozimego przy użyciu techniki RAPD. Folia Univ. Agric. Stetin., Agricultura 228 (91): 175 — 178.
Google Scholar
Tyrka M., Kociuba W. 2002. Ocena zróżnicowania genetycznego odmian pszenżyta ozimego 6x za pomocą zmodyfikowanej metody AFLP. Folia Univ. Agric. Stetin., Agricultura 228 (91): 185 — 190.
Google Scholar
Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids. Res. 18: 6531 — 6535.
Google Scholar
Wolko B., Bartkowiak-Broda I. 1997. Metody diagnostyki molekularnej w hodowli roślin [in: Hodowla roślin. Materiały z I Krajowej Konferencji], Poznań, 19–20 listopada 1997: 389 — 402.
Google Scholar
Authors
Aneta Kramekaneta.kramek@up.lublin.pl
Instytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie Poland
Statistics
Abstract views: 19PDF downloads: 24
License
Copyright (c) 2008 Aneta Kramek
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Wanda Kociuba, Aneta Kramek, Krzysztof Ukalski, Stability estimation of selected yielding traits of Polish winter triticale varieties , Bulletin of Plant Breeding and Acclimatization Institute: No. 264 (2012): Regular issue
- Roman Prażak, Wanda Kociuba, Zbigniew Segit, Aneta Kramek, Evaluation of the tolerance to salt stress of selected genotypes of spring durum wheat (Triticum durum Desf.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 283 (2018): Special issue
- Wanda Kociuba, Aneta Kramek, Intra-cultivar variability of useful traits of Polish winter triticale cultivars , Bulletin of Plant Breeding and Acclimatization Institute: No. 249 (2008): Regular issue
- Wanda Kociuba, Aneta Kramek, The value of hybrids of winter triticale with wheat in relation to the yielding traits and protein content in grain , Bulletin of Plant Breeding and Acclimatization Institute: No. 253 (2009): Regular issue
- Aneta Kramek, Krystyna Szwed-Urbaś, Zbigniew Segit, The estimation of variability and correlation of quantitative traits in spring durum wheat collection of Afghan origin , Bulletin of Plant Breeding and Acclimatization Institute: No. 264 (2012): Regular issue
- Aneta Kramek, Wanda Kociuba, Jacek Gawroński, The value of the new winter triticale cultivars in comparison to the regenerated collection materials , Bulletin of Plant Breeding and Acclimatization Institute: No. 283 (2018): Special issue
Similar Articles
- Irena Kolasińska, Diversity in fertility restoration among male sterile rye lines with Pampa cytoplasm , Bulletin of Plant Breeding and Acclimatization Institute: No. 264 (2012): Regular issue
- Dariusz Gozdowski, Danuta Martyniak, Wiesław Mądry, Multivariate evaluation of cultivars and advanced lines of perennial ryegrass in respect of traits important in seed production , Bulletin of Plant Breeding and Acclimatization Institute: No. 253 (2009): Regular issue
- Andrzej Latusek, Henryk Bujak, Evaluation of yield stability of winter oilseed rape cultivars on the basis of post-registration variety testing system in diversified soils and climatic conditions in Lower Silesia , Bulletin of Plant Breeding and Acclimatization Institute: No. 265 (2012): Regular issue
- Beata Myśków, Anna Łań, Monika Hanek, Analysis of the impact of pollination method on the results of mapping QTL for pre-harvest sprouting in rye , Bulletin of Plant Breeding and Acclimatization Institute: No. 264 (2012): Regular issue
- Tadeusz Drzazga, Jakub Paderewski, Paweł Krajewski, The estimation of winter wheat field response to variable environmental conditions in post-registration cultivar trials , Bulletin of Plant Breeding and Acclimatization Institute: No. 253 (2009): Regular issue
- Karolina Krystkowiak, Tadeusz Adamski, Maria Surma, Zygmunt Kaczmarek, Anetta Kuczyńska, Variation of wheat cultivars with regard to agronomic traits estimated by uni- and multivariate analysis , Bulletin of Plant Breeding and Acclimatization Institute: No. 253 (2009): Regular issue
- Krzysztof Wójcik, Rafał Kuriata, Sylwia Lewandowska, Heritability and relationships between maize agricultural traits , Bulletin of Plant Breeding and Acclimatization Institute: No. 264 (2012): Regular issue
- Roman Prażak, Morphological traits and fertility of hybrids of some Aegilops species with hexaploid wheat Triticum aestivum L. , Bulletin of Plant Breeding and Acclimatization Institute: No. 263 (2012): Regular issue
- Elżbieta Czembor, Marta Puchta, Urszula Piechota, Jerzy H. Czembor, Genetic diversity in the collection of modern and historical inbred lines of maize for resistance to ear rot caused by Fusarium verticillioides and the ability to accumulate fumonisins , Bulletin of Plant Breeding and Acclimatization Institute: No. 283 (2018): Special issue
- Kamila Nowosad, Henryk Bujak, Maize genotyping using KASP markers , Bulletin of Plant Breeding and Acclimatization Institute: No. 283 (2018): Special issue
<< < 9 10 11 12 13 14 15 16 17 18 19 > >>
You may also start an advanced similarity search for this article.
