Application of RAPD markers for fingerprinting of triticale cultivars
Paweł Milczarski
dziekanat.wksir@zut.edu.plKatedra Hodowli Roślin, Akademia Rolnicza w Szczecinie (Poland)
Aneta Banek-Tabor
Katedra Hodowli Roślin, Akademia Rolnicza w Szczecinie (Poland)
Piotr Masojć
Katedra Hodowli Roślin, Akademia Rolnicza w Szczecinie (Poland)
Abstract
Random amplified polymorphic DNA (RAPD) was used to study fingerprints and genetic similarities among 16 Polish cultivars of winter triticale. Polymorphic DNA fragments were sought for by testing of 50 arbitrary primers. Final studies were carried out using 17 selected primers that allowed for amplification of the useful 30 RAPD markers. This set of markers produced fingerprints for all the studied triticale cultivars and constituted a basis for developing a dendrogram presenting genetic similarities among varieties.
Keywords:
fingerprinting, genetic similarities, RAPD markers, triticale cultivarsReferences
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Authors
Paweł Milczarskidziekanat.wksir@zut.edu.pl
Katedra Hodowli Roślin, Akademia Rolnicza w Szczecinie Poland
Authors
Aneta Banek-TaborKatedra Hodowli Roślin, Akademia Rolnicza w Szczecinie Poland
Authors
Piotr MasojćKatedra Hodowli Roślin, Akademia Rolnicza w Szczecinie Poland
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