The comparison of amino acid composition of the protein in preparations obtained from potato tubers cultivated within ecological and conventional systems

Anna Pęksa

anna.peksa@upwr.edu.pl
Katedra Technologii Rolnej i Przechowalnictwa, Uniwersytet Przyrodniczy we Wrocławiu (Poland)

Elżbieta Rytel


Katedra Technologii Rolnej i Przechowalnictwa, Uniwersytet Przyrodniczy we Wrocławiu (Poland)

Agnieszka Tajner-Czopek


Katedra Technologii Rolnej i Przechowalnictwa, Uniwersytet Przyrodniczy we Wrocławiu (Poland)

Agnieszka Kita


Katedra Technologii Rolnej i Przechowalnictwa, Uniwersytet Przyrodniczy we Wrocławiu (Poland)

Maria Pytlarz-Kozicka


Katedra Szczegółowej Uprawy Roślin, Uniwersytet Przyrodniczy we Wrocławiu (Poland)

Joanna Miedzianka


Katedra Technologii Rolnej i Przechowalnictwa, Uniwersytet Przyrodniczy we Wrocławiu (Poland)

Abstract

The aim of the experiment was to study the influence of thermal coagulation of protein in potato juice isolated from tubers of three potato varieties cultivated conventionally and in ecological system on amino acid composition and nutritive value of obtained preparations. A soil fertilizer and copper salt 50 WP as a fungicide were used within ecological system of potato cultivation and in conventional system fertilizing with high doses of NPK, herbicides, insecticides and fungicides were applied. Protein coagulation was performed in juice containing dissolved CaCl2. After suitable hydrolysis of the obtained concentrates, the chemical composition and contents of 18 amino acids were determined in total protein with the use of automatic amino acid analyser. Potato protein concentrates (PPC) obtained from tubers cultivated within ecological system contained more nitrogen compounds and also forms of protein nitrogen than there were found in preparations originating from juice of tubers cultivated conventionally. Also, in amino acid composition of isolated protein we found more such essential amino acids as Leucine, Phenylalanine, Tyrosine and Threonine. Among remaining amino acids in PPC originating from potatoes cultivated within ecological system there was more of Asparagine acid, Serine and Glycine. Chemical Score (CS) expressing nutritive value of studied concentrates, in dependence of protein standard chosen for comparison occurred similar or higher than it was established for standard, particularly in the case of preparations obtained from tubers cultivated within ecological system.


Keywords:

concentrates, cultivation system, nutritive value, potato protein

Ahlden I., Trägårdh G. 1992. A study of soluble proteins from four potato varieties used in the Swedish starch industry. Food Chem. 44: 113 — 118.
Google Scholar

AOAC. 1990. W Herlich, K. (ed.). Official Methods of Analysis of AOAC: Food Composition; Additives; Natura contaminants. Vol. II. AOAC, Arlington.
Google Scholar

Büchi Kjeldahl line K — 422. 1998. Instrukcja obsługi aparatu.
Google Scholar

Ciećko Z., Krajewski W., Wyszkowski W., Wyszkowski M., Żołnowski A. 2004. Działanie nawożenia fosforowego na zawartość i jakość białka w bulwach ziemniaka. Prace Nauk. AE Wroc., Chemia 1017: 99 — 110.
Google Scholar

Danilchenko H., Pranaitiene R., Tarasieviciene Z., Venskutoniene. 2008. The effect of inhibitors on the amino acid content in the stored potato tubers. Zesz. Probl. Post. Nauk Rol. 530: 301 — 316.
Google Scholar

Eppendorfer W. H., Eggum B. O. 1994. Dietary fibre, starch, amino acids and nutritive value of potatoes as affected by sulfur, nitrogen, phosphorus, potassium, calcium and water stress. Acta Agric. Scand., Sect. B, Soil and Plant Sci.:107 — 115.
Google Scholar

Galdón B. R., Mesa D. R., Rodrígez E. M. R., Romero C. D. 2010. Amino acid content in traditional potato cultivars from the Canary Islands. J. Food Comp. Anal. 23: 148 — 153.
Google Scholar

Gawęcki J. 2011. Żywienie człowieka. Podstawy nauki o żywieniu. WNP. Warszawa: 210 — 215.
Google Scholar

Kapoor A. C., Desborough S. L., Li P. H. 1975. Potato tuber proteins and their nutritional quality. Potato Res. 18: 469 — 478.
Google Scholar

Knorr D. 1978. Protein quality of the potato and potato protein concentrates. Lebensm.-Wiss. u.-Technol. 11: 109 — 115.
Google Scholar

Knoor D. 1980. Effect of recovery methods on yield, quality and functional properties of potato protein concentrates. J. Food Sci. 45: 1183 — 1186.
Google Scholar

Knorr D., Kohler G. O., Betschart A. A. 1977. Potato protein concentrates: the influence of various methods of recovery upon yield compositional and functional characteristics. J. Food Process. Preserv. 2: 235 — 247.
Google Scholar

Leszczyński W. 2000. Jakość ziemniaka konsumpcyjnego. Żywn., Nauka, Technol., Jakość 4 (25) Supl.: 5 — 26.
Google Scholar

Lindner P., Kaplan B., Weiler E., Ben-Gera I. 1980/81. Fractionation of potato juice proteins into acid-soluble and acid-coagulable fractions. Food Chem. 6: 323 — 335.
Google Scholar

Lisińska G., Leszczyński W. 1972. Metodyka badań jakości bulw traktowanych w uprawie herbicydami ziemniaka jako surowca dla przemysłu spożywczego. Pr. Nauk. Inst. Technol. Org. i Tworz. Szt. P. Wroc. 9: 55 — 61.
Google Scholar

Løkra S., Helland M. H., Clausen I. C., Strætkvern K.O., Egelandsdal B. 2008. Chemical characterization and functional properties of a potato protein concentrate prepared by large-scale expanded bed adsorption chromatography. LWT 41: 1089 — 1099.
Google Scholar

Miedzianka J., Pęksa A., Aniołowska M. 2012. Properties of acetylated potato protein preparations. Food Chem. 133: 1283 — 1291.
Google Scholar

Mitrus J., Stankiewicz C., Steć E., Kamecki M., Starczewski J. 2003. The influence of selected cultivation on the content of total protein and amino acids in the potato tubers. Plant Soil Environ. 4 (3): 131 — 134.
Google Scholar

Moore S. 1963. On the determination of cystine as cysteic acid. J. Biol. Chem. 238: 235 — 237.
Google Scholar

Pęksa A. 2006. Ocena jakości preparatów białka ziemniaczanego otrzymanych w różnych warunkach technologicznych i ich przydatności w produkcji wyrobów ekstrudowanych. Zesz. Nauk. AR Wroc., Rozprawy, 533: 101.
Google Scholar

Pęksa A., Rytel E., Kita A., Lisińska G., Tajner-Czopek A. 2009. The properties of potato protein. Food 3 (Special Issue 1). Potato: Food, Nutrition and Health, Global Science Books: 79 — 87.
Google Scholar

PN-75/A-04018. Oznaczanie azotu metodą Kjeldahla i przeliczanie na białko.
Google Scholar

Ralet M. Ch., Gueguen J. 2000. Fractionation of potato proteins: solubility, thermal coagulation and emulsifying properties. Lebensm.-Wiss. u.-Technol. 33: 380 — 387.
Google Scholar

Seibles T. S. 1979. Studies on potato proteins. Am. Potato J. 56: 415 — 425.
Google Scholar

Spackman D. H., Stein W. H., Moore S. 1958. Automatic recording apparatus for use in the chromatography amino acid. Anal. Chem. 30: 1190 — 1206.
Google Scholar

Spies J. R., Chambers D. C. 1949. Chemical determination of tryptophan in proteins. Anal. Chem. 21: 1249 — 1266.
Google Scholar

Stankiewicz C., Bombik A., Rymuza K., Starczewski J. 2008. Wpływ wybranych zabiegów agrotechnicznych na zawartość białka oraz aminokwasów egzogennych w bulwach ziemniaka odmiany Irga i Ekra w trakcie ich przechowywania. Zesz. Probl. Post. Nauk. Rol. 530: 281 — 291.
Google Scholar

van Koningsveld G. A., Gruppen H., de Jongh H. H. J., Wijngaards G., van Boekel M. A. J. S., Walstra P., Voragen A. G. J. 2001. Effects of pH and heat treatments on the structure and solubility of potato proteins in different preparations. J. Agric. Food Chem. 49: 4889 — 4897.
Google Scholar

Wojnowska I., Poznański S., Bednarski W. 1981. Processing of potato protein concentrates and their properties. J. Food Sci. 47: 167 — 172.
Google Scholar

Wyszkowski M. 1996. Skład aminokwasowy białka bulw czterech odmian ziemniaka w zależności od nawożenia azotem. Acta Acad. Agricult. Tech. Olst., Agricult. 63: 129 — 138.
Google Scholar

Young V. R., Pellett P. L. 1991. Protein evaluation, amino acid scoring and the food and drug administration’s proposed food labeling regulations. Issues and Opinions in Nutrition. J. Nutr. 121: 145 — 150.
Google Scholar


Published
2012-12-31

Cited by

Pęksa, A. (2012) “The comparison of amino acid composition of the protein in preparations obtained from potato tubers cultivated within ecological and conventional systems”, Bulletin of Plant Breeding and Acclimatization Institute, (266), pp. 307–317. doi: 10.37317/biul-2012-0031.

Authors

Anna Pęksa 
anna.peksa@upwr.edu.pl
Katedra Technologii Rolnej i Przechowalnictwa, Uniwersytet Przyrodniczy we Wrocławiu Poland

Authors

Elżbieta Rytel 

Katedra Technologii Rolnej i Przechowalnictwa, Uniwersytet Przyrodniczy we Wrocławiu Poland

Authors

Agnieszka Tajner-Czopek 

Katedra Technologii Rolnej i Przechowalnictwa, Uniwersytet Przyrodniczy we Wrocławiu Poland

Authors

Agnieszka Kita 

Katedra Technologii Rolnej i Przechowalnictwa, Uniwersytet Przyrodniczy we Wrocławiu Poland

Authors

Maria Pytlarz-Kozicka 

Katedra Szczegółowej Uprawy Roślin, Uniwersytet Przyrodniczy we Wrocławiu Poland

Authors

Joanna Miedzianka 

Katedra Technologii Rolnej i Przechowalnictwa, Uniwersytet Przyrodniczy we Wrocławiu Poland

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Copyright (c) 2012 Anna Pęksa, Elżbieta Rytel, Agnieszka Tajner-Czopek, Agnieszka Kita, Maria Pytlarz-Kozicka, Joanna Miedzianka

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