Electrophoretic analysis of storage proteins in some winter triticale strains resistant to preharvest sprouting
Jacek Waga
j.waga@ihar.edu.plZakład Oceny Jakości i Metod Hodowli Zbóż, Instytut Hodowli i Aklimatyzacji Roślin w Krakowie (Poland)
Helena Grzesik
Zakład Oceny Jakości i Metod Hodowli Zbóż, Instytut Hodowli i Aklimatyzacji Roślin w Krakowie (Poland)
Abstract
The polymorphism of storage proteins was analyzed in some strains of winter triticale resistant to preharvest sprouting, in comparison with susceptible to sprouting variety Bogo. Based on the electrophoretic analysis differences in subunits composition of storage proteins were observed between the resistant to sprouting strains and the Bogo variety with regard to both gliadins and glutenins. These differences may be genetically associated with variability of the studied trait. It suggests a possibility of using them as biochemical markers of preharvest sprouting.
Keywords:
electrophoretic analysis, preharvest sprouting, storage proteins, triticaleReferences
Bushuk W., Zillman R. R. 1978. Wheat cultivar identification by gliadin electrophoregrams. I. Apparatus, method and nomenclature. Can. J. Plant Sci. 58: 505 — 515.
DOI: https://doi.org/10.4141/cjps78-076
Google Scholar
Derycke V., Haesaert G., Latre J., Struik P. C. 2002. Relation between laboratory sprouting resistance test and field observations in triticale (X Triticosecale Wittmack) genotypes. Proc. of 5th International Triticale Symposium, Radzików, Poland, vol. I: 123 — 133.
Google Scholar
Doliński R. 1995. Ocena polskich odmian uprawnych i rodów hodowlanych heksaploidalnego ozimego pszenżyta pod względem odporności na porastanie. Biul. IHAR 195/196: 147 — 158.
Google Scholar
Flinthan I. E., Gale M. D. 1982. The Tom Thumb dwarfing gene Rht3 in wheat. I Reduced preharvest sprouting damage to bread making quality. Theor Appl. Genet. 62: 121 — 126.
DOI: https://doi.org/10.1007/BF00293343
Google Scholar
Gale M. D., Salter A. M., Curtis F. C., Angus W. J. 1988. The exploitation of the Tom Thumb dwarfing gene, Rht3, in F1 hybrid wheats. IAEA-TECDOC — 455. Semi-dwarf cereal mutants and their use in cross-breeding III: 57 — 68.
Google Scholar
Grzesik H. 2002. Charakterystyka rodów pszenżyta ozimego odpornych na porastanie. Biul. IHAR 223/224: 145 — 150.
Google Scholar
Grzesik H., Gut M., Węgrzyn S., Cygankiewicz A. 2001. Związek porastania z innymi cechami heksaploidalnego pszenżyta ozimego (X Triticosecale Witt.). Biul. IHAR 218/219: 269 — 275.
Google Scholar
Grzesik H., Gut M., Węgrzyn S., Cygankiewicz A. 2003. Genetyczne uwarunkowania niektórych cech pszenżyta ozimego. Biul. IHAR 226/227: 227 — 231.
Google Scholar
Kączkowski J. 2002. Struktura i znaczenie jakościowe składników glutenu pszenicy — metody badawcze. Biul. IHAR 223/224: 3 — 31.
Google Scholar
Laemmli V. K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, 227: 680 — 685.
DOI: https://doi.org/10.1038/227680a0
Google Scholar
Morris R., Schmidt J. W., Jonson V. A. 1972. Chromosomal location of a dwarfing gene in "Tom Thumb" wheat derivative by monosomic analysis. Crop Sci. 12: 247 — 249.
DOI: https://doi.org/10.2135/cropsci1972.0011183X001200020032x
Google Scholar
Moś M. 1994. Porastanie pszenżyta ozimego. Zeszyty Naukowe Akademii Rolniczej w Krakowie, Rozprawy nr 195.
Google Scholar
Moś M. 2003. Changes in the germinability and vigour of winter triticale seeds with sprouting damage. Plant Soil Environ. 49, 3: 126 — 130.
DOI: https://doi.org/10.17221/4101-PSE
Google Scholar
Moś M., Wójtowicz T. 2002. Variability of field emergence of winter triticale seeds with sprouting damage. Cereal Res. Commun. 30, 3/ 4: 391 — 396.
DOI: https://doi.org/10.1007/BF03543434
Google Scholar
Payne P. I., Holt L. M., Jackson E. A., Law C. N. 1984. Wheat storage proteins: Their genetics and their potential for manipulation by plant breeding. Phil. Trans. R. Soc. London B 304: 259 — 371.
DOI: https://doi.org/10.1098/rstb.1984.0031
Google Scholar
Ruebenbauer T., Brej S. 1957. Hodowla roślin zbożowych. PWRiL, Warszawa.
Google Scholar
Rybka K., Małuszyńska E. 2002. Is the major pre-harvest sprouting resistance locus in triticale localized on the third rye chromosome? Proc. of 5th International Triticale Symposium, Radzików, Poland, vol. II: 219 — 222.
Google Scholar
Shishlova N. P., Shishlova A. M. 2002. Influence of abiotic factors on the resistance of winter triticale to pre-harvest sprouting in the conditions of Belarus. Proc. of 5th International Triticale Symposium, Radzików, Poland, vol. II: 223 — 228.
Google Scholar
Tarkowski C. 1978.Czynniki warunkujące produktywność roślin. PWN, Warszawa.
Google Scholar
Varshney R. K., Prasad M., Roy J. K., Roder M. S., Balyan H. S., Gupta P. K. 2001. Integrated physical maps of 2DL, 6BS and 7DL carrying loci for grain protein content and pre-harvest sprouting tolerance in bread wheat. Cereal Res. Commun. 29, 1/ 2: 33 — 40.
DOI: https://doi.org/10.1007/BF03543639
Google Scholar
Wahl T. I., O'Rourke A. D. 1992. The economics of sprout damage in wheat. In: Walker-Simmons M. K., Ried J. L. (eds.). Pre-harvest sprouting in cereals. USA: 3 — 7.
Google Scholar
Weidner S. 1992. Przedsprzętne porastanie ziarniaków zbóż i jego regulacja. Post. Nauk Rol. 5/ 6: 89 — 104.
Google Scholar
Węgrzyn S., Gut M., Cygankiewicz A., Ptak B. 1991. Odporność rodów i odmian pszenicy (Triticum aestivum L.) na porastanie. Część I. Pszenica ozima. Biul. IHAR 180: 121 — 129.
Google Scholar
Węgrzyn S., Gut M., Grzesik H., Bichoński A. Struś M. 1998. Resistance to pre-harvest sprouting and other traits of winter triticale. Proc. of 4th International Triticale Symposium, Red Deer, Alberta, Canada, vol. 2: 354—356.
Google Scholar
Zanetti S., Winzeler M., Keller M., Keller B., Messmer M. 2000. Genetic analysis of pre-harvest sprouting resistance in a wheat x spelt cross. Crop Sci. 40: 1406 — 1417.
DOI: https://doi.org/10.2135/cropsci2000.4051406x
Google Scholar
Zeleny L., Greenway W. T., Georgia M., Fifield C. C., Lelsbach K. L. 1960. Sedimentation values an index of dough mixing characteristics in early generation wheat selection. Cereal Chem. 37: 673 — 676.
Google Scholar
Authors
Jacek Wagaj.waga@ihar.edu.pl
Zakład Oceny Jakości i Metod Hodowli Zbóż, Instytut Hodowli i Aklimatyzacji Roślin w Krakowie Poland
Authors
Helena GrzesikZakład Oceny Jakości i Metod Hodowli Zbóż, Instytut Hodowli i Aklimatyzacji Roślin w Krakowie Poland
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