Separation of the fractions of potato juice of different molecular weight and characterization of their biological activities
Grażyna Lewandowicz
grazyna.lewandowicz@up.poznan.plKatedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Przemysław Kowalczewski
Katedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Wojciech Białas
Katedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Anna Olejnik
Katedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Joanna Rychlik
Katedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Abstract
The clinical trials on the medicinal use of potato juice proved its efficiency in the therapy of gastrointestinal disorders. However, the literature data don’t determine which substances actually reveal the therapeutic effect. The aim of the work was estimation of the biological activity of potato juice sprayed in the spray dryer, as well as its fractions of different molecular mass. Fresh potato juice was spray dried or alternatively, cryoconcentrated. As the result of the latter process fivefold concentrated potato juice has been obtained. This product was separated by means of gel permeation chromatography. The isolated fractions were analysed in terms of their capability to inhibit the proliferation and to reduce viability of human cancer cell lines. Human normal cell lines were used as a reference. Our studies proved that processed potato juice, similar to the fresh one, reveals cytotoxicity potential to stomach and colon cancer cells. The spray drying only slightly decreased biological activity of potato juice. It is possible to obtain fivefold concentrated potato by application of cryoconcentration process. That product could be effectively separated by means gel filtration process. The highest cytotoxicity, specifically targeted to the cancer cells, was revealed in non-protein, thermally stable fraction of molecular mass below 1 kDa.
Supporting Agencies
Keywords:
cytotoxicity, cryoconcentration, potato juice, separation, spray dryingReferences
Blum H., Beier H., Gross H. J.1987. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8: 93 — 99.
Google Scholar
Burlingame B., B Mouille B., Charrondiere R. 2009. Nutrients, bioactive non-nutrients and anti-nutrients in potatoes. Critical Review. J. Food Compos. Anal. 22: 494 — 502.
Google Scholar
Chrubasik S., Chrubasik C., Torda T., Madish A. 2006. Efficacy and tolerability of potato juice in dyspeptic patients: A pilot study. Phytomedicine 13: 11 — 15.
Google Scholar
Dembczyński R., Białas W., Biegańska-Marecik R., Jankowski T. 2009. Wpływ metody zagęszczania na właściwości wybranych soków owocowych. Aparatura Badawcza i Dydaktyczna 1: 63 — 69.
Google Scholar
Fellows P.J., 2000. Food Processing Technology, 2nd ed. Woodhead Publishing, Cambridge, England. Part II–IV.
Google Scholar
Jerzykiewicz J. 2007. Alkaloidy roślin z rodziny psiankowatych (Solanaceae). Postepy Biochem. 53 (3): 287 — 296.
Google Scholar
Ji Y.B., Gao S.Y., Ji C.F., Zou X. 2008. Induction of apoptosis in HepG2 cells by solanine and Bcl-2 protein.J. Ethnopharmacol. 115: 194 — 202.
Google Scholar
Korpan Y.I. Nazarenko E.A. Skryshevskaya, Martelet C., Jaffrezic-Renault N., El’skaya A.V. 2004. Potato glycoalkaloids: true safety or false sense of security. Trends Biotechnol. 22: 147 — 151.
Google Scholar
Kowalczewski P., Celka K., Białas W., Lewandowicz G. 2012. Antioxidant activity of potato juice. Acta Sci. Pol., Technol. Aliment. 11 (2): 175 — 181.
Google Scholar
Kuo K-W., Hsu S-H., Lin W-L., Liu L-F., Chang L-C. Lin C-C., Lin C-N., Sheu H-M. 2000. Anticancer Activity Evaluation of the Solanum Glycoalkaloid Solamargine, Biochem. Pharmacol. 60: 1865 — 1873.
Google Scholar
Lutaladio NB., Castaldi L. 2009. Potato: The hidden treasure. J. Food Compos. Anal. 22: 491 — 493.
Google Scholar
Løkra S., Schϋller R.B., Egelandsdal B., Engebretsen B., Straetkvern K.O. 2009. Comparison of composition, enzyme activity and selected functional properties of potato proteins isolated from potato juice with two different expanded bed resins. LWT — Food Sci. Technol. 42: 906 — 913.
Google Scholar
Miedzianka J., Pęksa A., Smolarczyk E. 2010. Zastosowanie przemysłowego soku ziemniaczanego do otrzymywania preparatów białka arylowanego. Zeszyty Problemowe Postępów Nauk Rolniczych 557: 261 — 273.
Google Scholar
Miedzianka J., Pęksa A., Aniołowska M. 2012. Properties of acetylated potato protein preparations. Food Chem. 133: 1283 — 1291.
Google Scholar
Mosnmann T. 1983. Rapid colorimetric assay for estimation for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65: 55 — 63.
Google Scholar
Nowotny F. 1972. Technologia przetwórstwa ziemniaczanego. Wydawnictwo Naukowo-Techniczne, Warszawa.
Google Scholar
Olejnik A, Białas W., Tomczyk J., Lewandowicz G. 2011. Cytotoksyczność i genotoksyczność soku z ziemniaka. Zeszyty Naukowe Uniwersytetu Ekonomicznego w Poznaniu nr 205: 118 — 125.
Google Scholar
Pastuszewska B., Taciak M., Tuśnio A. 2007. Koncentrat białka ziemniaczanego w żywieniu zwierząt monogastrycznych. Postępy Nauk Rolniczych 5: 91 — 106.
Google Scholar
Pęksa A., Gołubowska G., Rytel E., Lisińska G., Aniołowski K. 2002. Influence of harvest date on the glycoalkaloid contents of three potato varieties. Food Chem. 78: 313 — 317.
Google Scholar
Pęksa A., Gołubowska G., Aniołowski K., Lisińska G., Rytel E., 2006. Changes of glycoalkaloids and nitrate contents in potatoes during chip processing. Food Chem. 97: 151 — 156.
Google Scholar
Pouvreau L., Gruppen H., Piersma S. R., van den Broek A. M., van Koningsveld G.A., Voragen A. G. J. 2001. Relative Abundance and Inhibitory Distribution of protease inhibitors in potato juice from cv. Elkana. J. Agr. Food Chem. 49: 2864 — 2874.
Google Scholar
Ruseler-van Embden J. G. H., van Lieshout L. M. C., Laman J.D., 2004, Methods and means for preventing or treating inflammation or prurities. Patent USA 6: 723,354.
Google Scholar
Rytel E. 2010. Wybrane substancje odżywcze i antyżywieniowe ziemniaka i zmiany ich zawartości podczas przetwarzania na produkty spożywcze. Zeszyty Problemowe Postępów Nauk Rolniczych 557: 43 — 61.
Google Scholar
Saleem T.S,M., Chetty C.M., Ramkanth S., Alagusundaram M., Gnanaprakash K., Rajan V.S.T., Angalaparameswari S. 2009. Solanum nigrum Linn. — A review. Pharmacogn. Rev. 3 (6): 342 — 345.
Google Scholar
Tajner-Czopek A., Rytel E., Kita A., Pęksa A., Hamouz K. 2012. The influence of thermal process of coloured potatoes on the content of glycoalkaloids in the potato products. Food Chem. 133: 117 — 1122.
Google Scholar
Tuśnio A. Pastuszewska B. Swiech E. Taciak M. 2011. Response of young pigs to feeding potato protein and potato fibre — nutritional, physiological and biochemical parameters. J. Anim. Feed Sci. 20 (3): 361 — 378.
Google Scholar
Vlachojannis, J.E., Cameron M., Chrubasik S. 2010. Medicinal Use of Potato-derived Products, A Systematic Review. Phytother. Res. 24.
Google Scholar
Zgórska K., Czerko Z., Grudzińska M. 2006. Wpływ wybranych czynników na zawartość glikoalkaloidów w bulwach ziemniaka. Żyw. Nauk. Technol. Ja. 1 (46) Supl.: 229 — 234.
Google Scholar
Zwijnenbergh H.J. Kemperman A.J.B. Boerrigter M.E. Lotz M. Dijksterhuis J.F. Poulsen P.E., Klops G.-H. 2002. Native protein recovery from potato fruit juice by ultrafiltration. Desalination 144: 331 — 334.
Google Scholar
Authors
Grażyna Lewandowiczgrazyna.lewandowicz@up.poznan.pl
Katedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu Poland
Authors
Przemysław KowalczewskiKatedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu Poland
Authors
Wojciech BiałasKatedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu Poland
Authors
Anna OlejnikKatedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu Poland
Authors
Joanna RychlikKatedra Biotechnologii i Mikrobiologii Żywności, Uniwersytet Przyrodniczy w Poznaniu Poland
Statistics
Abstract views: 30PDF downloads: 25
License
Copyright (c) 2012 Grażyna Lewandowicz, Przemysław Kowalczewski, Wojciech Białas, Anna Olejnik, Joanna Rychlik
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Anna Bryła, Wojciech Juzwa, Grażyna Lewandowicz, Potato juice encapsulation into liposomes , Bulletin of Plant Breeding and Acclimatization Institute: No. 272 (2014): Regular issue
- Joanna Le Thanh-Blicharz, Jacek Anioła, Aleksander Walkowski, Grażyna Lewandowicz, Digestibility of diets containing RS modified starches assessment in rats , Bulletin of Plant Breeding and Acclimatization Institute: No. 266 (2012): Regular issue
- Przemysław Kowalczewski, Grażyna Lewandowicz, Agnieszka Makowska, Agata Olejnik, Wiktor Obuchowski, Characterisation of the extruded cereal snacks containing potato juice , Bulletin of Plant Breeding and Acclimatization Institute: No. 266 (2012): Regular issue
Similar Articles
- Dariusz R. Mańkowski, Biological progress in breeding, seed technology and production of potato in Poland. Part I. Review of quantitative methods applied to estimate of breeding and cultivar progress , Bulletin of Plant Breeding and Acclimatization Institute: No. 251 (2009): Regular issue
- Sławomir Wróbel, Barbara Robak, New potato cultivars characterized in regard to dormancy breaking of tubers , Bulletin of Plant Breeding and Acclimatization Institute: No. 272 (2014): Regular issue
- Lesław Juszczak, Sławomir Pietrzyk, Teresa Fortuna, Dorota Gałkowska, Teresa Witczak, Selected physicochemical properties of potato starch oxidized at different levels , Bulletin of Plant Breeding and Acclimatization Institute: No. 272 (2014): Regular issue
- Tomasz Erlichowski, Damage of tuber flesh by soil pests decreasing the value of table and processing potatoes , Bulletin of Plant Breeding and Acclimatization Institute: No. 273 (2014): Regular issue
- Dariusz R. Mańkowski, Zbigniew Laudański, Biological progress in breeding, seed technology and production of potato in Poland. Part VI. Assessment of biological progress on the basis of experiments and survey data , Bulletin of Plant Breeding and Acclimatization Institute: No. 254 (2009): Regular issue
- Lucyna Słomińska, Roman Zielonka, Leszek Jarosławski, Marek Buszka, Hydrolysis of non-gelatinized potato starch by using amylolytic enzymes , Bulletin of Plant Breeding and Acclimatization Institute: No. 266 (2012): Regular issue
- Teresa Witczak, Anna Stępień, Mariusz Witczak, Rheological stability of gels based on potato starch with the addition of pectin and inulin with different degree of polymerization , Bulletin of Plant Breeding and Acclimatization Institute: No. 272 (2014): Regular issue
- Krystyna Zarzyńska, Anna Wierzbicka, Magdalena Grudzińska, Organic potato production guarantees its health features , Bulletin of Plant Breeding and Acclimatization Institute: No. 279 (2016): Regular issue
- Dominika Boguszewska-Mańkowska, Potato soil drought resistance and assessment methods , Bulletin of Plant Breeding and Acclimatization Institute: No. 279 (2016): Regular issue
- Magdalena Grudzińska, Kazimiera Zgórska, Zbigniew Czerko, Effect of sprouting inhibitors on the colour of potato French fries , Bulletin of Plant Breeding and Acclimatization Institute: No. 265 (2012): Regular issue
<< < 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 > >>
You may also start an advanced similarity search for this article.
